Existence of ATP sensitive potassium currents on human periodontal ligament cells |
| |
| Affiliation: | 1. Department of Oral Physiology, School of Dentistry & Institute of Oral Bioscience, Chonbuk National University, Jeonju, South Korea;2. Department of Oral Biochemistry, School of Dentistry & Institute of Oral Bioscience, Chonbuk National University, Jeonju, South Korea;1. Department of Microbiology, College of Medicine, Korea University, Seoul 136-705, Korea;2. Department of Anatomy and Division of Brain Korea 21 Biomedical Science, College of Medicine, Korea University, Seoul 136-705, Korea;1. Material Measurement Laboratory, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899, USA;2. Volpe Research Center, American Dental Association Foundation, Gaithersburg, MD 20899, USA;3. X-ray Science Division, Argonne National Laboratory, 9700 S. Cass Avenue, Argonne, IL 60439, USA;1. Department of Physics, University of the Free State, Bloemfontein ZA9300, South Africa;2. Department of Physics and Electronics, Rhodes University, Grahamstown ZA6140, South Africa;3. School of Physics, Shri Mata Vaishno Devi University, Katra 182320, Jammu Kashmir, India;1. Advanced Materials Research Center, Materials Engineering Department, Najafabad Branch, Islamic Azad University, Najafabad, Iran;2. Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia;3. Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia;4. Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia |
| |
| Abstract: | ObjectivePotassium channels of the ATP-sensitive family (KATP channel) are inhibited by increase in intracellular ATP. Electrophysiological studies have demonstrated that the kinetics and pharmacological properties of KATP channels vary among different tissues, suggesting structurally and functionally distinct types. There are studies showing human periodontal ligament (PDL) cells respond to mechanical stress by increasing ATP release, which participates in bone resorption or bone homeostasis. So, in this study we investigated the existence of KATP channel subunit and their single channel properties in human periodontal ligaments.Materials & methodThe human PDL cells were isolated from healthy erupted third molar. For patch-clamp experiments, human PDL fibroblasts were seeded on 3.5 cm plastic dishes. The inside-out patch clamp recordings were performed under voltage clamp mode. Reverse transcriptase polymerase chain reaction (RT-PCR) was conducted to identify the channel subunits. All pair-wise comparisons were performed by Paired t-test. A P value <0.05 was considered significant.ResultsWe observed mRNA transcripts for Kir6.1, Kir6.2 and Sur2B subuits in the human PDL cells. In inside-out patch mode, the single channel conductance was 163 pS at symmetrical K+ concentration of 140 mM and inward rectification was seen in ATP-free bath solution. The reversal potential of the currents was found to be 0 mV at symmetrical concentration (140 mM) of K+ in bath solution. The single channel currents were almost blocked by adding 5 mM ATP in the bath solution. However, the currents were not blocked by 100 μM glibenclamide, a subunit specific KATP channel blocker.ConclusionsThese results indicate that human PDL cells express KATP channels subunit including Sur2B and Kir6.1 and Kir6.2 which are sensitive to ATP but insensitive to glibenclamide. |
| |
| Keywords: | Human periodontal ligament cell Inside-out patch |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
