Hydrogen peroxide induces cell proliferation and apoptosis in pulp of rats after dental bleaching in vivo: Effects of the dental bleaching in pulp |
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| Affiliation: | 1. Endodontics, São Paulo State University (Unesp), School of Dentistry, 16015-050, Araçatuba, SP, Brazil;2. Basic Science, São Paulo State University (Unesp), School of Dentistry, 16015-050, Araçatuba, SP, Brazil;3. Restorative Dentistry, São Paulo State University (Unesp), School of Dentistry, 16015-050, Araçatuba, SP, Brazil;1. Department of Radiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran;2. Department of Oral Medicine, Faculty of Dentistry, Babol University of Medical Science, Babol, Iran;3. Cellular and Biology Research Center, Babol University of Medical Science, Babol, Iran;4. Dental Materials Research Center, Faculty of Dentistry, Babol University of Medical Science, Babol, Iran;5. Department of Radiotherapy, Faculty of Medicine, Babol University of Medical Science, Babol, Iran;6. Department of Physiology and Cellular and Molecular Biology Research Center, Babol University of Medical Science, Babol, Iran;7. Faculty of Medicine, Babol University of Medical Science, Babol, Iran;8. Department of Pharmacology, Faculty of Medicine, Babol University of Medical Science, Babol, Iran;9. Department of Radiopharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran;1. Department of Restorative Dentistry, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil;2. The University of Oklahoma Health Sciences Center, Department of Restorative Sciences, Division of Dental Biomaterials, College of Dentistry, Oklahoma City, OK, United States;1. Centro de Química Estrutural, Instituto Superior Técnico, ULisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal;2. Centro de Investigação Interdisciplinar Egas Moniz, Instituto Superior de Ciências da Saúde Egas Moniz, Quinta da Granja, 2829-511 Caparica, Portugal;3. Department of Mechanical Engineering, University of Coimbra, R. Luis Reis Santos, 3030-788 Coimbra, Portugal;4. Department of Mechanical Engineering, Setúbal School of Technology, Instituto Politécnico de Setúbal, 2910-761 Setúbal, Portugal;5. Center of Physics and Engineering of Advanced Materials, Instituto Superior Técnico, ULisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal;1. Universidade Federal de Pernambuco, Recife, PE, Brazil;2. Universidade Estadual Paulista, UNESP, Araraquara, SP, Brazil;1. Graduate student, Graduate Prosthodontics, Department of Restorative Dental Sciences, Division of Prosthodontics, College of Dentistry, University of Florida, Gainesville, Fla;2. Research Associate, Department of Restorative Dental Sciences, Division of Prosthodontics, College of Dentistry, University of Florida, Gainesville, Fla;3. Clinical Associate Professor and Graduate Program Director, Graduate Prosthodontics, Department of Restorative Dental Sciences, Division of Prosthodontics, College of Dentistry, University of Florida, Gainesville, Fla;4. Statistician, Department of Neurosurgery, College of Medicine, University of Florida, Gainesville, Fla;5. Professor, Department of Restorative Dental Sciences, Division of Prosthodontics, College of Dentistry, University of Florida, Gainesville, Fla;6. Associate Professor, Department of Restorative Dental Sciences, Division of Prosthodontics, College of Dentistry, University of Florida, Gainesville, Fla |
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| Abstract: | ObjectiveThis study provides an in vivo evaluation of the inflammatory response, levels of cell proliferation and apoptosis, and the presence of necrosis after dental bleaching with two concentrations of hydrogen peroxide (H2O2).DesignWistar rats were divided into Control (placebo gel), BLUE (20% H2O2, 1 × 50 min), and MAXX (35% H2O2, 3 × 15 min) groups. At 2 and 30 days, the rats were killed (n = 10). The jaws were processed for histology analysis and PCNA and Caspase-3-cleaved immunohistochemistry, and data were submitted to the Mann-Whitney or ANOVA test (P < 0.05).ResultsAt 2 days, the MAXX group showed necrosis and the BLUE group revealed moderate inflammation on the occlusal third of the crown (P < 0.05). At 30 days, tertiary dentin had formed and there was an absence of inflammation. The level of cell proliferation was higher in the middle third of the BLUE group (P < 0.05), and cervical of MAXX at 2 days (P < 0.05), decreasing at 30 days. The apoptosis was present at 2 days, particularly in the cervical third of the crown in the bleached groups (P < 0.05), with a decrease only at 30 days in the BLUE group (P < 0.05).ConclusionsThe concentration of H2O2 influences effects on the pulp tissue, where a higher concentration of H2O2 can cause necrosis in the pulp and a prolonged effect within the apoptotic process; lower concentrations of H2O2 provide moderate inflammation, cell proliferation and apoptosis with a reduction of these processes over time. |
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| Keywords: | Hydrogen peroxide Pulp tissue Tooth bleaching Proliferating cell nuclear antigen Caspase 3 |
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