Efficacy of a mouthrinse based on hydroxyapatite to reduce initial bacterial colonisation in situ |
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| Institution: | 1. Clinic of Operative and Pediatric Dentistry, Medical Faculty Carl Gustav Carus, TU Dresden, Fetscherstr. 74, D-01307 Dresden, Germany;2. Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, University Hospital, Saarland University, Building 73, D-66421 Homburg/Saar, Germany;1. Cardiothoracic Centre, Guy''s and St Thomas'' Hospitals, London, UK;2. Department of Infection, University of Leeds, Leeds, UK;3. Leeds Teaching Hospitals National Health Service Trust, Leeds, UK;1. Department of Cardiology, Taunton and Somerset National Health Service (NHS) Trust, Taunton, Somerset, UK;2. Integrated Care Research, University of Surrey, Guildford, Surrey, UK;3. Department of Cardiology, Guy''s and St Thomas'' NHS Foundation Trust, London, UK;4. Division of Infectious Diseases, Mayo Clinic College of Medicine, Rochester, MN, USA;5. Department of Oral Medicine, Carolinas Medical Center, Charlotte, NC, USA;6. School of Clinical Dentistry, University of Sheffield, Sheffield, UK;1. São Paulo State University (Unesp), School of Dentistry, Araçatuba, Department of Pediatric Dentistry and Public Health, Rua José Bonifácio 1193, 16015-050, Araçatuba, SP, Brazil;2. LIEC-Department of Chemistry, Federal University of São Carlos (UFSCar), 13565-905, São Carlos, SP, Brazil;1. Faculty of Sustainability, Institute of Sustainable Chemistry, Leuphana University Lueneburg, Universitaetsallee. 1, C13, 21335 Lueneburg, Germany;2. Clinic of Operative and Pediatric Dentistry, Medical Faculty Carl Gustav Carus, TU Dresden, l̥Fetscherstr. 74, D-01307 Dresden, Germany;3. Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, University Hospital, Saarland University, Building 73, D-66421 Homburg/Saar, Germany;1. Universidade Católica Portuguesa, CBQF Centro de Biotecnologia e Química Fina—Associate Laboratory, Escola Superior Biotecnologia, Porto, Portugal;2. Chemistry Department, University College London, London, United Kingdom;3. Department of Materials and Ceramic Engineering/CICECO—Aveiro Institute of Materials, University of Aveiro, Aveiro, Portugal;4. School of Engineering and Materials Science, Queen Mary University of London, London, United Kingdom |
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| Abstract: | ObjectiveThe present in situ - investigation aimed to specify the impact of pure hydroxyapatite microclusters on initial bioadhesion and bacterial colonization at the tooth surface.DesignPellicle formation was carried out in situ on bovine enamel slabs (9 subjects). After 1 min of pellicle formation rinses with 8 ml of hydroxyapatite (HA) microclusters (5%) in bidestilled water or chlorhexidine 0.2% were performed. As negative control no rinse was adopted. In situ biofilm formation was promoted by the intraoral slab exposure for 8 h overnight. Afterwards initial bacterial adhesion was quantified by DAPI staining and bacterial viability was determined in vivo/in vitro by live/dead-staining (BacLight). SEM analysis evaluated the efficacy of the mouthrinse to accumulate hydroxyapatite microclusters at the specimens’ surface and spit-out samples of the testsolution were investigated by TEM.ResultsCompared to the control (2.36 × 106 ± 2.01 × 106 bacteria/cm2), significantly reduced amounts of adherent bacteria were detected on specimens rinsed with chlorhexidine 0.2% (8.73 × 104 ± 1.37 × 105 bacteria/cm2) and likewise after rinses with the hydroxyapatite testsolution (2.08 × 105 ± 2.85 × 105 bacteria/cm2, p < 0.001). No demonstrable effect of HA-particles on Streptococcus mutans viability could be shown. SEM analysis confirmed the temporary adsorption of hydroxyapatite microclusters at the tooth surface. Adhesive interactions of HA-particles with oral bacteria were shown by TEM.ConclusionHydroxyapatite microclusters reduced initial bacterial adhesion to enamel in situ considerably and could therefore sensibly supplement current approaches in dental prophylaxis. |
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| Keywords: | Hydroxyapatite Pellicle Bacteria Bioadhesion |
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