Effect of iRoot SP and mineral trioxide aggregate (MTA) on the viability and polarization of macrophages |
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| Affiliation: | 1. The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine of Ministry of Education (KLOBM), School and Hospital of Stomatology, Wuhan University, Wuhan, China;2. Department of Periodontology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China;3. Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China;1. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 31116, Republic of Korea;2. Department and Research Institute of Dental Biomaterials and Bioengineering, BK21 PLUS Project, College of Dentistry, Yonsei University, Seoul 03722, Republic of Korea;3. Department of Biomaterials Science, College of Dentistry, Dankook University, Cheonan 31116, Republic of Korea;4. Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 31116, Republic of Korea;5. Department of Microbiology, BK 21 Project for Medical Science, Institute for Immunology and Immunological Diseases, College of Medicine, Yonsei University, Seoul 03722, Republic of Korea;1. State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Conservative Dentistry and Endodontics, West China School and Hospital of Stomatology, Sichuan University, Chengdu, 610041, China;2. Department of Conservative Dentistry and Endodontics, School & Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325027, China;3. State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Orthodontics, West China School and Hospital of Stomatology, Sichuan University, Chengdu, 610041, China;4. Division of Endodontics, Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, Vancouver, British Columbia, Canada;1. Department of Conservative Dentistry, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea;2. Maxillofacial Tissue Regeneration and Research Center for Tooth and Periodontal Regeneration, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea;3. Pediatric Dentistry and School of Dentistry, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea;4. Department of Conservative Dentistry, Dental Research Institute and BK 21 Program, School of Dentistry, Seoul National University, Seoul, Republic of Korea;1. Department of Endodontics, School of Dentistry–Grande Rio University (UNIGRANRIO), Duque de Caxias, RJ, Brazil;2. Department of Endodontics, Rio de Janeiro State University (UERJ), Rio de Janeiro, RJ, Brazil;3. Department of Endodontics, Fluminense Federal University (UFF), Niterói, RJ, Brazil;4. Department of Periodontology, Veiga de Almeida University (UVA), Rio de Janeiro, RJ, Brazil;5. Department of Paediatric Dentistry, Fluminense Federal University (UFF), Nova Fibrurgo, RJ, Brazil;6. Private Practice Limited to Endodontics, Stuttgart, Germany;7. Department of Preventive and Community Dentistry, Rio de Janeiro State University (UERJ), Rio de Janeiro, RJ, Brazil |
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| Abstract: | ObjectiveThis study was performed to investigate the effect of iRoot SP and mineral trioxide aggregate (MTA) on the viability and polarization of macrophages.MethodsThe effect of iRoot SP and MTA on the viability of RAW 264.7 macrophages was tested using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay after 1 and 2 days of culture. The gene expression levels of interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), interleukin 10 (IL-10), interleukin 12p40 (IL-12p40) were measured by quantitative real time polymerase chain reaction (qRT-PCR) after stimulation of the RAW 264.7 macrophages with iRoot SP and MTA. The expression levels of CD11c and CD206 in RAW 264.7 macrophages were examined by immunofluorescence and flow cytometry after stimulation with iRoot SP and MTA. The data were analyzed by one-way analysis of variance and the Tukey test.ResultsBoth iRoot SP and MTA were non-toxic to the RAW 264.7 macrophages. The use of iRoot SP and MTA increased the expression of IL-1β, TNF-α, IL-10, IL-12p40 on the first day of culture and could promote macrophage M1 and M2 polarization.ConclusionsMTA and iRoot SP have good biocompatibility with macrophages, and they induced both M1 and M2 polarization of the RAW 264.7 macrophages. |
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| Keywords: | iRoot SP Mineral trioxide aggregate Viability Polarization RAW 264.7 macrophages |
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