The local administration of parathyroid hormone encourages the healing of bone defects in the rat calvaria: Micro-computed tomography,histological and histomorphometric evaluation |
| |
| Affiliation: | 1. Graduate Program in Dentistry, Universidade Positivo, R. Prof. Pedro Viriato Parigot de Souza, 5300 – Cidade Industrial, Curitiba, PR, 81280-330, Brazil;2. Departamento de Odontologia Restauradora, Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Avenida do Café, s/no – Campus da USP Ribeirão Preto, SP, CEP 14040-904, Brazil;1. Professor and HOD, Department of Oral and Maxillofacial Surgery, Institute of Dental Sciences, Pilibhit By Pass Road, Bareilly, Uttar Pradesh, India, 243006;2. Post Graduate Student, Department of Oral and Maxillofacial Surgery, Institute of Dental Sciences, Pilibhit By Pass Road, Bareilly, Uttar Pradesh, India, 243006;3. Professor, Department of Oral and Maxillofacial Surgery, Institute of Dental Sciences, Pilibhit By Pass Road, Bareilly, Uttar Pradesh, India, 243006;4. Reader, Department of Oral and Maxillofacial Surgery, Institute of Dental Sciences, Pilibhit By Pass Road, Bareilly, Uttar Pradesh, India, 243006;5. Senior Lecturer, Department of Oral and Maxillofacial Surgery, Institute of Dental Sciences, Pilibhit By Pass Road, Bareilly, Uttar Pradesh, India, 243006;1. UNESP - Univ. Estadual Paulista, Araçatuba Dental School, Department of Pediatric Dentistry and Public Health, Rua José Bonifácio, 1193, 16015050, Araçatuba, SP, Brazil;2. UNESP - Univ. Estadual Paulista, Araçatuba Dental School, Department of Restorative Dentistry, Rua José Bonifácio, 1193, 16015050, Araçatuba, SP, Brazil;3. UFRGS - Federal University of Rio Grande do Sul, Faculty of Dentistry, Department of Preventive Dentistry, Rua Ramiro Barcelos, 2492, Bairro Santa Cecília, 90035-003, Porto Alegre, RS, Brazil;4. UFRGS - Federal University of Rio Grande do Sul, Faculty of Dentistry, Department of Conservative Dentistry, Rua Ramiro Barcelos, 2492, Bairro Santa Cecília, 90035-003, Porto Alegre, RS, Brazil;1. Department of Dentistry, Universidade Positivo, Curitiba, Paraná, Brazil;2. Dental Clinic 1–Operative Dentistry and Periodontology, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany;3. Department of Dentistry, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil;3. School of Biological Sciences and Maurice Wilkins Centre for Molecular Biodiscovery, The University of Auckland, 3a Symonds Street, Auckland 1142, New Zealand;6. Department of Pathology and Pathogen Biology The Royal Veterinary College, Royal College Street, London NW1 0TU, United Kingdom;4. Departments of Biochemistry and Molecular Biology, Life Sciences Institute, The University of British Columbia, Vancouver V6T 1Z3, Canada;5. Departments of Microbiology and Immunology, Life Sciences Institute, The University of British Columbia, Vancouver V6T 1Z3, Canada;1. Institute for Research and Development, University of Vale do Paraíba, Av. Shishima Hifumi, 2911 São José dos Campos, SP, Brazil;2. School of Dentistry of Ribeirão Preto, University of São Paulo, Av. do Café, s/n, Ribeirão Preto, SP, Brazil;3. Materials Engineering Department, Federal University of São Carlos, UFSCar, Rod. Washington Luiz, km 235, São Carlos, SP, Brazil |
| |
| Abstract: | ObjectiveTo evaluate the effect of a single-dose local administration of PTH on bone healing in rat calvarial bone defects by means of micro-computed tomography, histological and histomorphometric analysis.DesignCritical-size cranial osteotomy defects were created in 42 male rats. The animals were randomly divided into 3 groups. In the C Group, the bone defect was only filled with a blood clot. In the S Group, it was filled with a collagen sponge and covered with bovine cortical membrane. In the PTH Group, the defect was filled with a collagen sponge soaked with PTH and covered with bovine cortical membrane. The groups were further split in two for euthanasia 15 and 60 days post-surgery. Data was statistically analyzed with t-tests for independent samples or the nonparametric Mann-Whitney test when applicable. Intragroup comparisons were analyzed with paired t-tests (p < 0.05).ResultsMicro-CT analysis results did not demonstrate statistically significant intergroup differences. At 15 days post-surgery, the histomorphometric analysis showed that the PTH Group exhibited a significantly higher percentage of bone formation compared with the S Group. At 60 days post-surgery, a higher percentage of new bone was observed in the PTH group.ConclusionThe results suggest that the local administration of PTH encouraged the bone healing in critical-size calvarial defects in rats. |
| |
| Keywords: | Parathyroid hormone Bone regeneration Rats Calvaria Micro-CT |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
