De novo synthetic short antimicrobial peptides against cariogenic bacteria |
| |
| Institution: | 1. State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China;2. The First People’s Hospital of Yunnan Province, Kunming, China;1. Department of Materials Science and Technology, Graduate School of Science and Technology, Niigata University, Niigata 950-2181, Japan;2. Graduate School of Technology, Niigata Institute of Technology, Niigata 945-1195, Japan;1. Department of Maxillofacial Surgery, Nihon University Graduate School of Dentistry at Matsudo, Chiba, Japan;2. Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan;1. State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China;2. Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China;3. Biomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA;4. National Engineering Research Centre for Biomaterials, Sichuan University, Chengdu 610064, China;1. Groupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval, 2420, rue de la Terrasse, Québec, G1V 0A6, QC, Canada;2. Unité IMEC, Faculté des Sciences de Bizerte, Unversité de Carthage, Tunisia;3. Forschungszentrum Borstel, Leibniz-Zentrum für Medizin und Biowissenschaften, Parkallee, D-23845, Borstel, Germany;4. Département de Chirurgie, Faculté de Médecine, Université Laval, and L''Axe Médecine régénératrice, Centre de recherche du CHU de Québec, Québec, G1L 3L5, Canada;5. Brandenburg Antiinfektiva GmbH, c/o Forschungszentrum Borstel, D-23845, Borstel, Germany;1. Laboratory for the Study of Regenerative Dental Medicine, Department of Oral Histology and Developmental Biology, Dental Research Institute and School of Dentistry, Seoul National University, 1 Gwanakro, Gwanak-gu, Seoul, South Korea;2. Regenerative Dental Medicine R & D Center, HysensBio Co., Ltd., 10 Dwitgol-ro, Gwacheon-si, Gyeonggi-do, South Korea;3. Department of Conservative Dentistry, Dental research Institute and School of Dentistry, Seoul National University, Jongno-gu, Seoul, South Korea |
| |
| Abstract: | ObjectiveAntimicrobial peptides (AMPs) have shown the ability to inhibit planktonic bacteria and biofilms. The objectives of this study were to de novo design and synthesize a series of cationic, amphipathic α-helical AMPs that would be shorter, less cytotoxic, and more potent than existing AMPs against cariogenic bacteria.DesignThree short AMPs (GH8, GLLWHLLH-NH2; GH12, GLLWHLLHHLLH-NH2; and GH16, GLLWHLLHHLLHLLHH-NH2) were designed, synthesized and characterized structurally. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) against eight major cariogenic bacteria were tested to select the most promising peptide. Scanning electron microscopy (SEM) was used to observe the bacterial membrane after treatment with selected peptides. The bactericidal kinetics, effects on biofilm and cytotoxity were further investigated.ResultsOf the three AMPs, GH12 had the most balanced structural parameters and a high content of α-helical structure. GH12 had a MIC of 4.0-8.0 μg/mL and MBC of 8.0-32.0 μg/mL. The corresponding values for the other two AMPs were 2- to 64- fold higher. In time-kill assays, GH12 killed all bacterial strains within 60 min at 4- fold MBC. SEM observed lysis and pore formation of the cytomembrane after treatment with GH12. 8.0 μg/mL GH12 inhibited Streptococcus mutans biofilm formation. Confocal laser scanning microscopy showed that GH12 effectively reduced the biomass of 1-day-old S. mutans biofilm. Cytotoxicity assays indicated that GH12 showed little toxic effect on the viability of human gingival fibroblasts.ConclusionThese results indicate that GH12 shows antimicrobial activity against cariogenic bacteria and biofilms in vitro. |
| |
| Keywords: | Antimicrobial peptide Dental caries Antimicrobial activity Biofilms |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
