| 基于DNA条形码的玉叶金花属植物鉴定研究 |
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| 引用本文： | 龚维,陈湜,刘婉桢,邓小芳,涂铁要.基于DNA条形码的玉叶金花属植物鉴定研究[J].中草药,2015,46(5):727-732. |
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| 作者姓名： | 龚维 陈湜 刘婉桢 邓小芳 涂铁要 |
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| 作者单位： | 华南农业大学生命科学学院, 广东 广州 510642;福建农林大学益虫研究所, 福建 福州 350002;华南农业大学生命科学学院, 广东 广州 510642;国家林业局管理干部学院, 北京 102600;中国科学院华南植物园 中国科学院植物资源保护与可持续利用重点实验室, 广东 广州 510650 |
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| 基金项目： | 国家自然科学基金资助项目(31300174);教育部博士点基金项目(20124404120014 |
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| 摘 要： | 目的利用DNA条形码进行玉叶金花属植物的分子鉴定。方法对玉叶金花属20种、89份个体进行叶绿体片段rbc L、mat K和trn H-psb A以及核基因片段ITS的PCR扩增和测序,计算种内和种间Kimura 2-parameter(K2-P)遗传距离,利用序列相似性法和邻接法进行单一片段、核心片段、组合片段以及ITS2片段的分析。结果单一片段分析结果表明玉叶金花属种间遗传距离(0.002~0.012)明显大于种内遗传距离(0.000 3~0.000 7)。trn H-psb A扩增率最低,ITS2片段的分辨率最高,其次是mat K和ITS片段,rbc L片段的分辨率最低。片段组合后的分辨率明显提高,基于序列相似性法和邻接法,mat K+rbc L+ITS和mat K+rbc L+trn H-psb A+ITS的分辨率相当,且比其他片段组的分辨率更高,分别为77%和75%,成功鉴定了15个近缘类群,包括2个药用种类广西玉叶金花和黐花。结论鉴于trn H-psb A扩增率较低,建议mat K+rbc L+ITS作为玉叶金花属物种鉴定的DNA条形码,并作为其药用种类的分子鉴定工具。
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| 关 键 词： | 玉叶金花属 DNA条形码 物种鉴定 mat K trn H-psb A rbc L ITS ITS2 |
| 收稿时间： | 2014/9/19 0:00:00 |
Identification of plants in Mussaenda L. based on DNA barcoding |
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| GONG-Wei,CHEN-Shi,LIU Wan-zhen,DENG Xiao-fang and TU Tie-yao.Identification of plants in Mussaenda L. based on DNA barcoding[J].Chinese Traditional and Herbal Drugs,2015,46(5):727-732. |
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| Authors: | GONG-Wei CHEN-Shi LIU Wan-zhen DENG Xiao-fang and TU Tie-yao |
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| Institution: | College of Life Sciences, South China Agricultural University, Guangzhou 510642, China;Institute of Beneficial Insects, Fujian Agriculture and Forestry University, Fuzhou 350002, China;College of Life Sciences, South China Agricultural University, Guangzhou 510642, China;Institute of Cadre, State Academy of Forestry Administration, Beijing 102600, China;Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China |
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| Abstract: | Objective This study aims at developing fast and accurate species identification methods for the plants of Mussaenda L. Methods In the present study, DNA barcoding analysis was carried out on 89 individuals representing 20 species of Mussaenda in order to evaluate the performance of the four candidate barcoding loci (matK, rbcL, trnH-psbA, and ITS) and ITS2 region. Based on sequence similarity and Neighbor-joining (NJ) tree reconstruction, we detected inter- and intra-specific genetic distances using Kimura 2-parameter (K2P). Results Inter-specific genetic distance of species in Mussaenda was significantly higher than intra-specific genetic distance. The region of ITS2 showed the highest discrimination power among the independent sequences. Comparably high species discrimination power was also revealed by the matK and ITS data set. The candidate barcode of rbcL displayed the lowest identification rate among the others. However, each individual candidate barcode demonstrated significantly lower discrimination power than the barcode of combined data set. Comparable discrimination power was revealed between the two barcodes of combined sequences matK + rbcL + ITS and matK + rbcL + trnH-psbA + ITS, which showed the values around 77% and 75% based on sequence similarity and NJ tree method. Totally 15 species were identified based on NJ analysis of matK + rbcL + ITS. Conclusion Consequently, the combined sequence of matK + rbcL + ITS provides an effective and fast tool for the identification and authentication of medicinal plant species in the genus Mussaenda L. |
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| Keywords: | Mussaenda L DNA barcoding species identification matK trnH-psbA rbcL ITS ITS2 |
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