| 冠状动脉内给予乙酰半胱氨酸对缺血再灌注心肌梗死面积的影响 |
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| 引用本文： | 陶贵周,张川海.冠状动脉内给予乙酰半胱氨酸对缺血再灌注心肌梗死面积的影响[J].临床心血管病杂志,2010,26(1). |
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| 作者姓名： | 陶贵周 张川海 |
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| 作者单位： | 辽宁医学院附属第一医院心内科,辽宁锦州,121001 |
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| 基金项目： | 辽宁省教育厅高等学校科研项目基金资助(No:2008408) |
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| 摘 要： | 目的:观察冠状动脉内给予小剂量N-乙酰半胱氨酸(NAC)对家兔心肌缺血再灌注(I/R)后心肌梗死面积的影响。方法:36只家兔随机分为假手术组(S组,12只)、(I/R组,12只)和NAC治疗组(NAC组,12只)。NAC组在结扎前降支1h后恢复血流灌注,然后用无损伤血管钳夹闭主动脉和肺动脉10s,同时于心尖部注入200g/L的NAC溶液0.5ml,S组和I/R组给予0.5ml0.9%氯化钠溶液。再灌注3h后从颈动脉采血,测定血清肌酸磷酸激酶(CK)、乳酸脱氢酶(LDH)的含量和谷胱甘肽过氧化物酶(GPH-Px)活性。蛋白印迹法检测缺血区心肌组织中I-κB的表达,并分别用双重染色测定心肌梗死面积。结果:与I/R组比较,NAC组CK和LDH明显降低(P<0.01),而GPH-Px活性则明显增高(P<0.01),缺血区心肌组织中I-κB的表达明显减少,心肌梗死面积也显著减少(P<0.01)。结论:冠状动脉内给予小剂量NAC能明显减少心肌I/R后的心肌梗死面积。
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| 关 键 词： | 再灌注损伤 N-乙酰半胱氨酸 谷胱甘肽过氧化物酶 核因子-κappaB |
Effects of N-acetylcysteine administrated by coronary artery on infarct size fter myocardial ischemia-reperfusion |
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| TAO Guizhou,ZHANG Chuanhai.Effects of N-acetylcysteine administrated by coronary artery on infarct size fter myocardial ischemia-reperfusion[J].Journal of Clinical Cardiology,2010,26(1). |
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| Authors: | TAO Guizhou ZHANG Chuanhai |
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| Institution: | Department of Cardiology/a>;the First Affiliated Hospital of Liaoning Medical University/a>;Jinzhou/a>;121001/a>;China |
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| Abstract: | Objective:To evaluate the effects of low dose N-acetylcysteine (NAC) given through coronary artery on infarct size in a rabbit myocardial ischemia-reperfusion model. Method:Thirty-six rabbits were randomly divided into sham operation group(S group,n=12),myocardial ischemia/reperfusion group (I/R group,n=12) and the NAC treatment group (NAC group,n=12). After one hour's occlusion of anterior descending artery,the blood flow were restored of NAC group,then occluded the aorta and pulmonary artery 10 s with non... |
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| Keywords: | N-acetylcysteine reperfusion injury glutathione peroxidase |
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