一氧化氮合酶在膀胱癌中的表达及临床意义

目的：探讨一氧化氮（NO）在膀胱肿瘤中的作用及临床意义。方法：采用免疫组织化学方法对58例膀胱移行细胞癌标本（实验组）及14例良性膀胱组织标本（对照组）进行一氧化氮合酶（NOS）抗体染色，观察表达结果与肿瘤生物学特性之间的相关性。结果：诱导型NOS（iNOS)在实验组中的阳性表达率明显高于对照组（P＜0．01），其表达程度与肿瘤分级、分期无关（P＞0．05）；内皮型NOS（eNOS）在实验组和对照组血管内皮细胞都有表达，但前者表达较强；而在对照组中多呈不连续的表达。结论：NO在膀胱移行细胞癌的发生、发展中起重要的作用。

Nitric oxide synthases expression in human bladder cancer and their clinical significance

Purpose: To evaluate NOS expression in bladder tissue from patients with transitional cell carcinoma (TCC) of the bladder.Methods:Bladder carcinoma tissue specimens were procured from 58 patients with TCC and 14 cases of benign tissue as control group. NOS immunohistochemistry were performed on all tissue specimens. Results: Inducible NOS specific proteins were found in 47 of 58 bladder cancer specimens but not in control bladder tissue. The malignant cells and inflammatory cells within the carcinomas were highly iNOS positive, specimens of bladder mucosa outside the malignant regions showed only a weak positive iNOS immunostaining. The endothelial cells in both normal urothelium and tumor tissue showed a highly positive eNOS immunostaining but its immunoreaction was not detected in either malignant or benign epithelium. A correlation between iNOS immunoreactivity and tumor grade in bladder carcinoma could not be verified.Conclusions:NOS play an important role in the biological effect of bladder carcinoma.