| HPLC法测定阿胶养颜软胶囊中维生素E的含量 |
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| 引用本文： | 田守生,郭尚伟,段小波,李士栋,郝向慧,尤金花. HPLC法测定阿胶养颜软胶囊中维生素E的含量[J]. 齐鲁药事, 2011, 30(7): 397-398 |
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| 作者姓名： | 田守生 郭尚伟 段小波 李士栋 郝向慧 尤金花 |
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| 作者单位： | 山东东阿阿胶股份有限公司,山东省胶类中药研究与开发重点实验室,山东,聊城,252201 |
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| 摘 要： | 目的建立维生素E含量的高效液相色谱法测定方法。方法以甲醇为溶剂,采用超声波提取制备供试品,色谱柱为Agilent Zorbax-C18(250 mm×4.6 mm,5μm),流动相为甲醇,检测波长284.6 nm,流速1.0 mL.min-1,柱温35℃。结果维生素E在0.053 1~0.424 4 mg.mL-1范围内线性良好,r=0.999 9;平均回收率为101.19%(n=9,RSD=2.06%)。结论此方法操作简便、快速准确,可用于阿胶养颜软胶囊等产品的维生素E含量测定。
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| 关 键 词： | 高效液相色谱法 超声提取 阿胶养颜软胶囊 维生素E 含量测定 |
Determination of Vitamin E in E-jiao Yangyan Soft Capsules by HPLC |
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| TIAN Shou-sheng,GUO Shang-wei,DUAN Xiao-bo,LI Shi-dong,HAO Xiang-hui,YOU Jin-hua. Determination of Vitamin E in E-jiao Yangyan Soft Capsules by HPLC[J]. qilu pharmaceutical affairs, 2011, 30(7): 397-398 |
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| Authors: | TIAN Shou-sheng GUO Shang-wei DUAN Xiao-bo LI Shi-dong HAO Xiang-hui YOU Jin-hua |
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| Affiliation: | (Shandong Dong′e E-jiao Co.,Ltd.,Shandong Key Laboratory for Research and Development of Animal Glue Medicine,Liaocheng 252201,China) |
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| Abstract: | Objective To establish an HPLC method for the determination of Vitamin E in E-jiao Yangyan Soft Capsules.Methods Vitamin E was extracted by methanol with ultrasonic extraction.Agilent Zorbax-C18 column（250 mm×4.6 mm,5 μm） was used.The mobile phase was methanol with the flow rate of 1.0 mL·min-1.The detection wavelength was 284.6 nm and the column temperature was 35 ℃.Results There was a good linearity in the range of 0.053 1~0.424 4 mg·mL-1（r=0.999 9）;The average recovery was 101.19% with RSD=2.06%.Conclusion The method was simple,fast and accurate,and it can be used for the determination of Vitamin E in E-jiao Yangyan Soft Capsules. |
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| Keywords: | HPLC Ultrasonic extraction E-jiao Yangyan Soft Capsules Vitamin E Determination |
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