小鼠肥大细胞瘤P815模型的建立与初步应用

目的 建立小鼠肥大细胞瘤Ｐ８１５种植瘤模型，并对其体内诱发特异性ＣＴＬ应答机理进行初步的研究。方法 经有限稀释法筛选Ｐ８１５单克隆瘤系，用ＲＴ－ＰＣＲ及产物ＤＮＡ测序鉴定肿瘤抗原Ｐ１Ａ基因的表达；在此基础上，用活瘤苗免疫同系小鼠，经标准＾５１Ｃｒ释放试验检测在体特异性ＣＴＬ的活性。结果 筛选出了一个Ｐ８１５单克隆成瘤系Ｐ８１－Ｆ３，并鉴定该瘤存在Ｐ１Ａ基因的表达，用活瘤疫苗免疫６只步鼠，在３只体内

The establishment of mouse mastocytoma P815 model and it's application in cellular immune trials

ObjectiveTo establish a mouse mastocytoma P815 model and make a preliminary study on the induction of specific CTL responses in this model. MethodsWe screened a monoclone P815 cell line by limiting dilution assay (LDA),and detected whether the cell line expresses gene P1A with RT PCR and DNA sequencing. Then DBA/2 mice were immunized with the living P815 cells and monitored the specific CTL responses by 51 Cr release assay. ResultsWe have selected a cell line P815 F3 and identified the expression of P1A gene in this cell line;after immunization with the living P815 F3 cells,the anti P815AB specific CTL responses could be detected in 3/6 mice.ConclusionAfter establishing the P815 tumor model,we make a preliminary study on the induction of specific CTL responses in vivo ,proving that immunization with the living P815 cells could initiate anti P815AB specific CTL responses in about 50% mice.