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| 热休克蛋白90对抗氯化钴引起的心肌细胞损伤 | |
| 引用本文： | 李建平,杨春涛,杨战利,廖新学,谭钰嫔,黄雪,王礼春,陈培熹,冯鉴强.热休克蛋白90对抗氯化钴引起的心肌细胞损伤[J].热带医学杂志,2009,9(8). |
| 作者姓名： | 李建平 杨春涛 杨战利 廖新学 谭钰嫔 黄雪 王礼春 陈培熹 冯鉴强 |
| 作者单位： | 1. 惠东县人民医院内科,惠东,516300 2. 中山大学中山医学院生理教研室,广州,510080 3. 中山大学附属第一医院,广州,510080 4. 南华大学附属湖南省人民医院,衡阳,421001 |
| 基金项目： | 广东省科技计划资助项目(No.2006B60501024,No.2007B080701030) |
| 摘 要： | 目的探讨热休克蛋白90(heat shock protein 90,HSP90)在对抗氯化钴(cobalt chloride,CoCl2)诱导H9C2心肌细胞损伤中的作用。方法应用不同浓度的CoCl2处理H9C2心肌细胞,建立化学性缺氧诱导心肌细胞损伤的实验模型。应用CCK-8比色法检测细胞存活率;Western-blot法检测HSP90的表达;双氯荧光素染色荧光显微镜照相检测细胞内活性氧(reactive oxygen species,ROS)水平;超氧化物歧化酶(superoxidedismutase,SOD)检测试剂盒分析SOD活性抑制率。结果在400～1000μmol/L浓度范围内,CoCl2处理H9C2心肌细胞36 h,呈剂量依赖性地抑制细胞存活率。在0.5～36 h时间范围内,600μmol/L CoCl2呈时间依赖性地促进H9C2心肌细胞HSP90的表达。2～16μmol/L HSP90抑制剂17-丙烯胺基-17-去甲氧基格尔德霉素(17-allylamino-17demethoxygeldanamycin,17AAG)呈剂量依赖性地加重600μmol/L CoCl2对H9C2细胞存活率的抑制。... |
| 关 键 词： | 热休克蛋白 氯化钴 心肌细胞 活性氧 |
Heat Shock Protein90 Protects H9C2 Cardiac Cells Against CoCl2-induced Injuries |
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| LI Jian-ping,YANG Chun-tao,YANG Zhan-li,LIAO Xin-xue,TAN Yu-pin,HUANG Xue,WANG Li-chun,CHEN Pei-xi,FENG Jian-qiang.Heat Shock Protein90 Protects H9C2 Cardiac Cells Against CoCl2-induced Injuries[J].Journal Of Tropical Medicine,2009,9(8). | |
| Authors: | LI Jian-ping YANG Chun-tao YANG Zhan-li LIAO Xin-xue TAN Yu-pin HUANG Xue WANG Li-chun CHEN Pei-xi FENG Jian-qiang |
| Institution: | 1.Department of Internal Medicine;Huidong People's Hospital;Huidong 516300;2.Department of Physiology;Zhongshan Medical College;Sun Yat-sen University;Guangzhou 510080;3.Department of Cardiovasology;the First Affiliated Hospital;4.Hunan Provincial People's Hospital;South China University;Hengyang 421001;China |
| Abstract: | Objective To investigate the protection of heat shock protein 90(HSP90)against H9C2 cardiac cell injuries induced by cobalt chloride(CoCl2).Method H9C2 cells were treated with CoCl2 at different concentrations to set up the chemical hypoxia-induced cardiomyocyte injury model.Cell viability was measured by using cell counter kit(CCK-8).The expression of HSP90 was tested by Western-blot assay.Intracellular reactive oxygen species(ROS)was evaluated by 2',7'-dichlorfluorescein-diacetate(DCFH-DA) staining and ph... |
| Keywords: | heat shock protein cobalt chloride cardiomyocyte reactive oxygen species |
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