肾癌患者外周血中碳酸酐酶9 mRNA表达的临床意义

目的：探讨肾细胞癌患者外周血中碳酸酐酶9（MN）mRNA表达的临床意义。方法：应用RT－PCR技术，对30例肾细胞癌患者及30例非肾细胞癌患者外周血中肾癌细胞标志物MN mRNA进行检测。结果：非肾癌组外周血中均不表达MN mRNA。肾癌患者外周血MN mRNA阳性表达13例（43％），其中肾癌转移8例，局限性肾癌5例；肾透明细胞癌12例，混合型癌1例。外周血MN mRNA表达与肾癌的临床分期无相关性（P＞0．05）；而肾透明细胞癌阳性率（12／22）明显高于其他组织类型（P＜0．05）。结论：RT－PCR技术可较敏感地检测到血液中肾癌细胞，有助于早期诊断肾癌微小转移。MN可被视为透明细胞癌特异的相关蛋白。

Clinical significance of MN mRNA expression in peripheral blood of renal cell carcinoma patients

Objective To study the expression of MN mRNA in peripheral blood of renal cell carcinoma(RCC) patients and to evaluate its clinical significance. Methods Peripheral blood MN mRNA expression was detected by means of RT PCR 30 patients with renal cell carcinoma,including 16 with localized RCC and 14 with metastatic RCC,in 30 patients without renal tumors series as controts. Results With RT PCR, MN mRNA expressin was detected in 13 of 30 cases of RCC (43%) including 5 of 16 patients(31%) with localized RCC and 8 of 14 patients(57%) with metastatic RCC, none of the 30 control cases being positive. No correlation was noted between MN results and tumor stage. Conclusions RT PCR assay of MN is a highly sensitive technique for detecting circulating renal carcinoma cells in the peripheral blood, and it may be helpful in the diagnosis of micrometastasis.MN can be regarded as a cell specific relative protein of clear cell renal carcinoma.