| CEA抗原表位串联体与FL共表达基因疫苗抑制小鼠肝癌细胞体内生长的研究 |
| |
| 引用本文： | 段秀梅,车媛媛,黄晶,石旭,李丹,刘力华,方艳秋,谭岩,宋燕.CEA抗原表位串联体与FL共表达基因疫苗抑制小鼠肝癌细胞体内生长的研究[J].中国免疫学杂志,2010,26(4). |
| |
| 作者姓名： | 段秀梅 车媛媛 黄晶 石旭 李丹 刘力华 方艳秋 谭岩 宋燕 |
| |
| 作者单位： | 1. 吉林大学第一医院中心实验室,长春,130021
2. 吉林大学中日联谊医院普通外科,长春,130033 |
| |
| 基金项目： | 吉林省科技厅重点项目 |
| |
| 摘 要： | 目的:观察CEA抗原表位串联体与FL共表达基因疫苗抑制荷瘤小鼠肿瘤生长。方法:利用基因重组技术将CEA抗原表位三串联体和FL基因片段克隆到质粒pcDNA3.0上,肌注免疫BALB/c小鼠,观察重组基因疫苗对CEA阳性肿瘤的抑制作用、小鼠生存时间及其诱导小鼠杀伤效应细胞的活性。结果:pcDNA-triCEA625-667-sFL免疫组小鼠生存时间延长,肿瘤生长速度缓慢,瘤块较小,与对照组小鼠比较有显著性差异(P0.01);经pcDNA-triCEA625-667-sFL免疫的小鼠脾细胞对H22-CEA+的杀伤率明显升高,差异有显著性(P0.01)。结论:共表达三倍体CEA抗原表位和FL的基因疫苗可以有效抑制CEA阳性肿瘤在小鼠体内的生长,并增强小鼠杀伤效应细胞的活性。
|
| 关 键 词： | 癌胚抗原 Flt3配体 抗原表位 基因疫苗 |
The inhibitory effects of the DNA vaccine co-expressing CEA tandem repeat epitopes and FL on the in vivo growth of heptocarcinomas in mice |
| |
| DUAN Xiu-Mei,CHE Yuan-Yuan,HUANG Jing,SHI Xu,LI Dan,LIU Li-Hua,FANG Yan-Qiu,TAN Yan,SONG Yan.The inhibitory effects of the DNA vaccine co-expressing CEA tandem repeat epitopes and FL on the in vivo growth of heptocarcinomas in mice[J].Chinese Journal of Immunology,2010,26(4). |
| |
| Authors: | DUAN Xiu-Mei CHE Yuan-Yuan HUANG Jing SHI Xu LI Dan LIU Li-Hua FANG Yan-Qiu TAN Yan SONG Yan |
| |
| Institution: | DUAN Xiu-Mei,CHE Yuan-Yuan,HUANG Jing,SHI Xu,LI Dan,LIU Li-Hua,FANG Yan-Qiu,TAN Yan,SONG Yan.Central Laboratory of the First Hospital,Jilin University,Changchun 130021,China |
| |
| Abstract: | Objective:To observe inhibitory effects of DNA vaccine co-expressing CEA tandem repeat epitopes and FL on cancer cells in mice.Methods:The encoding sequences for CEA tandem repeats and FL were inserted into plasmid pcDNA3.0 using gene recombinant technique.BALB/c mice were immunized intramuscularly with the co-expressing DNA vaccine.The survival time and tumor size were measured and specific CTL cytotoxicity was detected by 125 I-UdR release method.Results:Compared with that of the control,the survival time... |
| |
| Keywords: | Carcinoembryonic antigen Flt3 ligand Antigen epitope Gene vaccine |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
