The central clock controls the daily rhythm of <Emphasis Type="Italic">Aqp5</Emphasis> expression in salivary glands |
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| Authors: | Hitoshi Uchida Takahiro J Nakamura Nana N Takasu Aya Obana-Koshino Hitomi Ono Takeshi Todo Takayoshi Sakai Wataru Nakamura |
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| Institution: | 1.Department of Oral-Chrono Physiology,Nagasaki University Graduate School of Biomedical Sciences,Nagasaki,Japan;2.Department of Oral-Facial Disorders, Graduate School of Dentistry,Osaka University,Suita,Japan;3.Department of Life Sciences, School of Agriculture,Meiji University,Kawasaki,Japan;4.Faculty of Pharmaceutical Sciences,Teikyo Heisei University,Tokyo,Japan;5.Department of Radiation Biology and Medical Genetics, Graduate School of Medicine,Osaka University,Suita,Japan |
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| Abstract: | Salivary secretion displays day–night variations that are controlled by the circadian clock. The central clock in the suprachiasmatic nucleus (SCN) regulates daily physiological rhythms by prompting peripheral oscillators to adjust to changing environments. Aquaporin 5 (Aqp5) is known to play a key role in salivary secretion, but the association between Aqp5 and the circadian rhythm is poorly understood. The aim of our study was to evaluate whether Aqp5 expression in submandibular glands (SMGs) is driven by the central clock in the SCN or by autonomous oscillations. We observed circadian oscillations in the activity of period circadian protein homolog 2 and luciferase fusion protein (PER2::LUC) in cultured SMGs with periodicity depending on core clock genes. A daily rhythm was detected in the expression profiles of Aqp5 in SMGs in vivo. In cultured SMGs ex vivo, clock genes showed distinct circadian rhythms, whereas Aqp5 expression did not. These data indicate that daily Aqp5 expression in the mouse SMG is driven by the central clock in the SCN. |
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