肝康栓抗鸭乙型肝炎病毒和改善肝脏病理的作用

目的]用鸭乙型肝炎（乙肝）模型研究肝康栓抗鸭乙型肝炎病毒（DHBV）和改善肝脏病理的作用。方法]用DHBV阳性血清感染1d龄的樱桃谷雏鸭，制备鸭乙肝模型。将其中36只感染阳性鸭随机分成3组：肝康栓治疗组，0．85％氯化钠模型组和阿昔洛韦（ACV）对照组，每组12只，均连续给药4周。分别于给药前，给药14、28d和停药7d时取血清，用实时定量PCR法检测鸭血清中DHIWDNA拷贝数。于停药7d时，处死各组雏鸭，取肝脏病理切片苏木精一伊红染色后，观察肝脏炎症情况和肝细胞变性程度。结果]肝康栓治疗组给药14、28d及停药7d时鸭血清中DHBVDNA拷贝数的对数值均较给药前降低（均P〈0.01）。肝康栓治疗组雏鸭肝脏的肝细胞肿胀率、空泡变率、嗜酸性变率均较同期模型组降低，差异有统计学意义（P〈0.05，〈20．01）。结论]肝康栓能有效地抑制鸭体内DHBVDNA的复制，并具有改善肝脏病理的作用。

Inhibitory effects of Gankang suppository on hepatitis B virus and ameliorating hepatic pathology in ducklings

Objective]To study the antiviral effect of Gankang suppository(GS) on duck hepatitis B virus(DHBV) in vivo,and observe the changes of hepatic pathology.Methods] One-day-old Yingtaogu ducklings infected with positive serum of DHBV were used as the hepatitis B animal model.Thirty-six positive infected ducklings were randomly divided into 3 groups(n=12,each) and administered continuously for 4 weeks: GS group,0.85 % saline model group,and acyclovir(ACV) control group.The changes of serum DHBV-DNA copies were detected by the method of real time PCR after the ducklings were treated for 14 days and 28 days,and 7 days after withdrawal.The pathological changes in liver tissue were observed 7 days after withdrawal. Results]After medicated with GS 14 days,28 days and 7 days after withdrawal,logarithmic value(log) of DHBV-DNA in ducklings' sera was obviously lower than that before treatment with significant difference(P<0.01).The hepatocellular hydropic degeneration rate,fatty degeneration rate and acidophilic degeneration rate in GS group were obviously lower than those of 0.85 % saline model group respectively with significant difference(P<0.05,P<0.01). Conclusion] GS could ameliorate hepatic pathology change in DHBV-infected ducklings via inhibiting the replication of DHBV-DNA.