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| 一测多评法测定丹参中丹参酮ⅡA、隐丹参酮、丹参酮Ⅰ、二氢丹参酮Ⅰ的含量 | |
| 引用本文： | 李倩,刘伟,罗祖良,杨美华. 一测多评法测定丹参中丹参酮ⅡA、隐丹参酮、丹参酮Ⅰ、二氢丹参酮Ⅰ的含量[J]. 中国中药杂志, 2012, 37(6): 824-828 |
| 作者姓名： | 李倩 刘伟 罗祖良 杨美华 |
| 作者单位： | 1. 北京协和医学院药用植物研究所中草药物质基础与资源利用教育部重点实验室,北京100193;河南中医学院,河南郑州450008 2. 河南中医学院,河南郑州,450008 3. 北京协和医学院药用植物研究所中草药物质基础与资源利用教育部重点实验室,北京100193;广西中医学院,广西南宁530001 4. 北京协和医学院药用植物研究所中草药物质基础与资源利用教育部重点实验室,北京,100193 |
| 基金项目： | 国家"重大新药创制"科技重大专项(2009ZX09502-025);国家中医药管理局2008年度中医药行业科研专项(200807042) |
| 摘 要： | 目的:建立一测多评法同时测定丹参中丹参酮ⅡA、丹参酮I、二氢丹参酮I、隐丹参酮的含量,验证该方法在丹参药材中应用的准确性和可行性。方法:以丹参药材为研究对象,药材中4个有效成分为指标性成分,分别建立丹参酮ⅡA与二氢丹参酮I、隐丹参酮、丹参酮I的相对校正因子,计算二氢丹参酮I、隐丹参酮、丹参酮I的含量,实现一测多评。同时采用外标法测定药材中4个指标性成分的含量,比较计算值与实测值的差异,以验证一测多评法的准确性和可行性。结果:16批丹参中4个指标性成分,采用校正因子计算的含量值与外标法实测值之间无显著差异。结论:在对照品缺乏的情况下,以外标法测定丹参酮ⅡA,利用相对校正因子实现对二氢丹参酮I、隐丹参酮、丹参酮I的含量测定是可行的,一测多评法可以用于丹参中多种成分的质量评价研究。 |
| 关 键 词： | 一测多评法 相对校正因子 丹参 |
| 收稿时间： | 2011-07-06 |
Simultaneous determination of four tanshinones in Salvia miltiorrhiza by QAMS |
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| LI Qian,LIU Wei,LUO Zuliang and YANG Meihua. Simultaneous determination of four tanshinones in Salvia miltiorrhiza by QAMS[J]. China Journal of Chinese Materia Medica, 2012, 37(6): 824-828 | |
| Authors: | LI Qian LIU Wei LUO Zuliang YANG Meihua |
| Affiliation: | Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine of Ministry of Education, Institute of Medicinal Plant Development, Peking Union Medical College, Beijing 100193, China;Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine of Ministry of Education, Institute of Medicinal Plant Development, Peking Union Medical College, Beijing 100193, China;Guangxi Traditional Chinese Medical University, Nanning 530001, China;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine of Ministry of Education, Institute of Medicinal Plant Development, Peking Union Medical College, Beijing 100193, China |
| Abstract: | Objective: To establish a new method and validate its feasibilities for quality evaluation of Salvia miltiorrhiza.Method: Four main effective components,dihydrotanshinone I,cryptotanshinone,tanshinone Ⅰ and tanshinone ⅡA,were selected as analytes to evaluate the quality of S.miltiorrhiza.The relative correction factors(RCF) of tanshinone ⅡA to the other three tanshinones were calculated.The method was evaluated by comparison of the quantitative results between external standard method and QAMS method.Result: No significant differences were found in the quantitative results of four tanshinones by external standard method and QAMS method.Conclusion: It is a convenient and accurate method to determine multi-components when some authentic standard substances were unavailable.It can be used to control the quality of S.miltiorrhiza. |
| Keywords: | quantitative analysis of multi-components by single marker (QAMS) relative correction factor (RCF) Salvia miltiorrhiza. |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |