长期外用狗皮膏对大鼠肾功能及铅蓄积的影响

目的:观察长期外用狗皮膏对大鼠肾功能影响及铅在肾脏蓄积情况。方法:长期(90 d)外用给予7,3.5,1.75 g.kg-13个剂量狗皮膏,检测给药45,90 d肾脏指数,血清尿素(BU),肌酐(Cr),尿β2-微球蛋白(β2-MG),N-乙酰-β-D-氨基葡萄糖苷酶(NAG)含量,并对肾脏做病理学检查;采用原子吸收光谱法测定肾脏铅的含量。结果:狗皮膏连续外用90 d高、中剂量组大鼠肾脏指数增高,高剂量组尿NAG增高,给药组肾脏铅含量明显高于空白对照组,而BU,Cr,β2-MG,肾病理学无明显改变。结论:在本剂量范围内,狗皮膏连续高剂量外用90 d可使大鼠肾脏指数增高、铅在肾脏蓄积、尿液NAG增高,但未影响到肾脏的排泄能力和发生器质性改变。

Effects of long-term external use of Goupi Gao on renal functions and lead accumulation in rats

Objective: To observe the effect of long-term external use of Goupi Gao on renal function and lead accumulation in rats.Method: Rats were externally administered with Goupi Gao at different doses(7,3.5 and 1.75 g·kg-1) for 90 d.At 45 days and 90 days after administration,the renal indicator,levels of blood urea nitrogen(BU) and creatinine(Cr) in serum,β2-microglobulin(β2-MG) and N-acety1-β-D-glucosaminidase(NAG) in urine were determined.Lead content in kidneys was detected by atomic absorption spectrometry.Result: A 90-day administration with Goupi Gao significantly enhanced the renal indicator,levels of NAG in urine and lead content in renal,when compared with the normal rats.However,the levels of BUN and β2-MG as well as renal pathology in Goupi Gao treated rats were not obviously changed.Conclusion: Consecutive administration of Goupi Gao for 90 days can increase the renal indicator and levels of NAG in urine,enhance the accumulation of lead in renal,but with no effect on excretory function of kidneys and organic changes.