紫金砂药材的指纹图谱研究

目的:建立紫金砂药材的高效液相指纹图谱方法。方法:采用Kromasil 100A C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-水为流动相梯度洗脱,流速为1 mL.min-1,检测波长为254 nm。以异欧前胡素为参照物,对10批紫金砂药材进行指纹图谱分析。结果:建立了紫金砂的HPLC指纹图谱共有模式,标定了8个共有峰,10批药材的相似度均>0.9。结论:该方法简便、准确、重复性好,为紫金砂药材的品质评价和质量控制标准的制定提供科学依据。

Fingerprint of Angelica polymorpha by HPLC

Objective: To establish the HPLC fingerprint of Angelica polymorpha.Method: The 10 batches of A.polymorpha were measured by HPLC with isoimperatorin as a reference substance and the chromatographic experients were performed on Kromasil 100A C18 column(4.6 mm × 250 mm,5 μm),eluted with acetonitrile and water as mobile phase in gradient mode.The flow rate was 1.0 mL·min-1 and the detection wavelength was 254 nm.Result: The common mode of the HPLC fingerprints were set up.There were 8 common peaks in the fingerprint of 10 samples,and the similarity of the 10 samples was more than 0.9.Conclusion: The method is simple,accurate and have a good reaptability.The quality of A.polymorpha can be controlled effectively by the HPLC fingerprint.