狗皮膏大鼠长期毒性试验的体内血液铅变化研究

目的:建立火焰原子吸收光谱法测定血铅方法,测定狗皮膏长期毒性试验的大鼠血铅浓度动态变化规律,为狗皮膏的安全性用药提供参考。方法:连续外用给予大鼠高、中、低剂量(7,3.5,1.75 g.kg-1)狗皮膏90 d,于给药前、给药后10,30,45,52,60,90 d及停药后16,28 d取血,采用微波消解仪消解血液样品,用石墨炉原子吸收光谱法测量血铅含量。结果:经方法学考察本次试验的标准曲线回归方程A=0.004 9X+0.017,r=0.999 5,检测限为0.380μg.L-1,精密度检查RSD 1.4%,混合血样中血铅值175.77μg.L-1,RSD 6.0%。大鼠给予狗皮膏后,血铅浓度逐渐升高,给药中、低剂量30 d后及高剂量10 d后血铅浓度达到稳定,给药量与血铅浓度具有剂量依赖性,停药后血铅浓度降低。结论:本方法测定准确可靠,适合于血铅的测定,狗皮膏长期大剂量外用可引起血铅升高。

Study on blood lead of rats in long-term toxicity test with Goupi Gao

Objective: To observe dynamic changes of blood lead concentration in rats with long-term toxicity test with Goupi Gao by the flame atomic absorption spectrometry,in order to provide reference for safe administration of Goupi Gao.Method: The rats were administered with Goupi Gao by high-dose(7 g·kg-1),medium-dose(3.5 g·kg-1),low-dose(1.75 g·kg-1) by external use for consecutively 90 days.Then,the blood samples were collected from the rats before the administration and at 10,30,45,52,60,90 d after the administration respectively,as well as 16 d and 28 d after the drug withdrawal.The samples were dispelled with microwave digestion system and then were determined by graphite furnace atomic absorption spectrometry for blood lead levels.Result: According to methodological study,the standard curve regression equation in this experiment was A=0.004 9X+0.017,r=0.999 5,with the detection limit up to 0.380 μg·L-1.The RSD was 1.4% by precision checks.Blood lead level of mixed blood samples was 175.77 μg·L-1,whose RSD was 6.0%.Blood lead concentration gradually increased after low-dose and medium-dose administration to rats and became stable at the 10th day and the 30th day by high-dose.Dose is directly related to blood lead concentration.Meanwhile,the blood lead concentration decreases after the drug withdrawal.Conclusion: The method of determination in this experiment is so accurate and reliable that it can be used for the determination of blood lead.Long-term and high-dose external use of Goupi Gao can increase blood lead.