| 改性心室辅助装置材料的细胞毒性及溶血性试验 |
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| 引用本文： | 刘春广,王轩,陈庆良.改性心室辅助装置材料的细胞毒性及溶血性试验[J].华北煤炭医学院学报,2010,12(4):459-461. |
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| 作者姓名： | 刘春广 王轩 陈庆良 |
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| 作者单位： | 1. 天津医科大学宝坻临床学院心脏科,天津,301800
2. 天津市胸科医院心外科 |
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| 基金项目： | 天津市科技发展计划项目 |
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| 摘 要： | ①目的通过细胞毒性试验和溶血性试验来筛选符合生物相容性要求的心室辅助装置材料。②方法 5种受试聚氨酯材料,制成60 cm23份,每份加入生理盐水注射液。取3支试管,每管加入生理盐水注射液作为阴性对照。取3支试管,每管加入蒸馏水作为阳性对照。全部试管置恒温水浴中保温,每支试管中加入稀释兔血,继续保温,吸出管内液体,离心后取上清液,测定其吸光度。上述材料灭菌后制备其浸提液,在含L-929细胞的培养皿的琼脂表面放置滴有材料浸提液的无菌滤纸片,培养箱避光培养后染色,最后在倒置相差显微镜下观测。③结果先接枝聚氨酯（APCU）、聚碳酸聚氨酯（PCU）、聚氨酯（PU）3种材料溶血率为0.3%、1.4%、0%,而先磺化聚氨酯（SPCU）及肝素化聚氨酯（HPU）溶血率为23.6%、12.1%;先接枝聚氨酯及聚碳酸聚氨酯毒性分级为0.6级和0.2级,而先磺化聚氨酯、肝素化聚氨酯及聚氨酯毒性分级为1.5级、1.5级和1.2级。④结论先接枝聚氨酯,聚碳酸聚氨酯和聚氨酯3种材料符合医用材料的溶血试验要求,先接枝聚氨酯及聚碳酸聚氨酯符合材料细胞毒性试验要求。
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| 关 键 词： | 聚氨酯 浸提液 生物相容性 溶血试验 细胞毒性试验 琼脂覆盖法 |
Cytotoxicity and hemolysis tests on modification materials of ventricular assisted devices |
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| LIU Chunguang,WANG Xuan,CHEN Qingliang.Cytotoxicity and hemolysis tests on modification materials of ventricular assisted devices[J].Journal of North China Coal Medical College,2010,12(4):459-461. |
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| Authors: | LIU Chunguang WANG Xuan CHEN Qingliang |
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| Institution: | LIU Chunguang,WANG Xuan,CHEN Qingliang(Department of Cardiology,Baodi Clinical College,Tianjin Medical University,Tianjin 301800,China) |
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| Abstract: | Objective To select the biocompatible requirements materials of the ventricular assisted device by cytotoxicity test and hemolysis test.Methods Five kinds of materials of polyurethane(APCU,PCU,PU,SPCU and HPCU)were divided into 3 parts(60cm2),sodium chloride injection was added in each of materials(60cm2).Sodium chloride injection was and added in three test-tubes as negative control and distilled water was added in three test-tubes as positive control.All of the test-tubes were placed in constant temperatu... |
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| Keywords: | Polyurethane Extract liquid Biocompatiblity Hemolysis test Cytotoxicity test Agar overlay test |
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