A simple method for obtaining cultures enriched for Type II pneumonocytes from fetal rabbit |
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| Authors: | Thomas B. Shea L. Darwin Keichline |
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| Affiliation: | (1) Department of Biology, Northeastern University, 02115 Boston, Massachusetts, USA |
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| Abstract: | Summary A differential plating method permitted preparation of cultures significantly enriched for Type II pneumocytes. These cells were maintained in a differentiated state for at least 12 d, identifiable morphologically (by presence of osmiophilic lamellar inclusion bodies) and bio-chemically (by demonstration of synthesis of phosphatidyl choline and production of disaturated lecithin). |
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| Keywords: | fetal lung Type II cells culture |
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