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| Characteristics of Ex Vivo Expansion of Endothelial Progenitor Cells | |
| 作者姓名： | 刘超 孙宗全 吴永超 陈新忠 冯建鄂 |
| 作者单位： | Department of Cardiac Surgery The Affiliated First Hospital of Zhengzhou University，Zhengzhou 450052，China，Department of Cardiac Surgery，Union Hospital，Tongji Medical College，Huazhong Uni-versity of Science and Technology，Wuhan 430022，China，Department of Orthopaedic Surgery，Union Hospital，Tongji Medical College，Huazhong Uni-versity of Science and Technology，Wuhan 430022，China，Department of Cardiac Surgery，Union Hospital，Tongji Medical College，Huazhong Uni-versity of Science and Technology，Wuhan 430022，China，Department of Cardiac Surgery，Union Hospital，Tongji Medical College，Huazhong Uni-versity of Science and Technology，Wuhan 430022，China |
| 基金项目： | This project was supported by a grant from Hubei Provin-cial Bureau of Health (No. WI01532). |
| 摘 要： | Recently, endothelial progenitor cells (EPCs) were iso- lated from peripheral blood and proved to participate in neovascular formation1-3]. This will be useful in regener- ating therapeutic vasculogenesis, provide suitable coat- ing for vascular grafts or deliver lethal toxins to tumor vascular beds. But EPCs in peripheral blood is very lim- ited in number, its introduction into transplantation pro- tocols has been hampered by the limited number of pro- genitors contained in blood harvested… |
| 关 键 词： | 内皮干细胞 细胞凋亡 膨胀性能 基因表达 |
| 收稿时间： | 2005-03-03 |
Characteristics of Ex Vivo expansion of endothelial progenitor cells |
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| Chao Liu,Zongquan Sun,Yongchao Wu,Xinzhong Chen,Jian’e Feng.Characteristics of Ex Vivo Expansion of Endothelial Progenitor Cells[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2006,26(4):411-413. | |
| Authors: | Chao Liu Zongquan Sun Yongchao Wu Xinzhong Chen Jian’e Feng |
| Institution: | 1. Department of Cardiac Surgery, The Affiliated First Hospital of Zhengzhou University, Zhengzhou 450052, China 2. Department of Cardiac Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 3. Department of Orthopaedic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
| Abstract: | Summary The characteristics for the ex vivo expansion of the endothelial progenitor cells (EPCs) were explored. CD34+ cells were selected from umbilical cord blood mononuclear cells (MNC) by MiniMACS system, expanded under the same conditions as those for total MNC, coincubation of CD34+ and CD34− from the same donor for EPCs. In addition, the effects of vessel endothelial growth factor (VEGF) and passage on cell differentiation, expansion kinetics and apoptosis were examined. EPCs were determined and quantified by immunocytochemistry and flow cytometry. The results showed that both coculture of CD34+ and CD34− and total MNC led to a significant increase in the expansion of CD34+ cells as compared with CD34 enrichment (P<0.05). There was a tendency toward decreased apoptosis in cultures when early passage was performed immediately after cord like structures appeared. VEGF had no significant effect on apoptosis (P>0.05). These differentiated EPCs were positive for CD34+, von Willebrand factor (vWF), KDR, CD31 staining and phagocytized acetylated low-density lipoprotein (LDL). CD34+ cells accounted for (68.2±6.3) % of attaching (AT) cells at day 7 of culture. It was suggested the most efficient method to ex vivo expansion of EPCs was coculture of CD34+ and CD34− or total MNC. Early passage makes cell apoptosis rate decrease. VEGF had no significant effect on ex vivo expansion of EPCs. LIU Chao, male, born in 1971, M.D., Ph.D. This project was supported by a grant from Hubei Provincial Bureau of Health (No. WI01532). |
| Keywords: | endothelial progenitor cell expansion CD34 apoptosis |
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