甲基丙烯酸改性甲基丙烯酸β羟乙酯水凝胶义眼台的实验研究

目的研究兔眶内植入甲基丙烯酸(MMA)改性甲基丙烯酸β羟乙酯(PHEMA)水凝胶义眼台的生物相容性和纤维血管化情况,为其临床应用奠定实验基础。方法将直径为14 mm的MMA改性PHEMA水凝胶义眼台分别手术植入32只兔眼眶内,分别于术后2、4、8、12及24周做单光子发射型计算机体层摄影术(SPECT)检查,并在不同时间点(2、4、8、12及24周)取出植入物进行光镜、免疫组化(Actin、Vimentin、CD34)及电镜观察。结果 SPECT检查改性PHEMA植入物在在体情况下随时间延长,同位素示踪剂在局部逐渐浓集。光镜、免疫组化、电镜检查结果显示2周时纤维血管组织从植入物外周长入,4～8周孔隙内胶原纤维逐渐致密,至12周全部纤维血管化。32例中仅1例术后出现结膜裂开、义眼台暴露,经二次手术修补愈后好。结论经改性的PHEMA水凝胶义眼台组织相容性好、血管化快,手术操作简单,并发症少,是一种安全、实用的新型眶内植入材料。

Biocompatibility of a special hydrogel orbital implant (PHEMA and MMA) in rabbits

Objective To investigate the biocompatibility of a hydrogel orbital implant and its rate of vascularization in an experimental study Methods The implant was made of copolymerization of 2 hydroxyethyl methacryate (HEMA) and methyl methacrylate (MMA) Thirty two hydrogel orbital implants were implanted into the right orbit of 32 New Zealand albino rabbits The rate of vascularization was examined by SPECT, immunohistochemistry and electron microscopy Results Single Positron Emission Computerized Tomography (SPECT) examination showed that a radiotracer could be found locally concentrated on hydrogel orbital implants in vivo along with time On light microscopy, implants fibro vascular tissue invade the pores of the hydrogel orbital implant after 2 weeks and gradually invaded the implant deeper and deeper from 4 to 8 weeks, almost all implants were fully vascularized after 12weeks with a relatively spare inflammatory reaction Only one case needed additional surgery Conclusions The hydrogel orbital implant has many advantages, such as well biocompatible, fast vascularization, simple operation and low complication of surgery It is safe and applicable for clinical use as a new type of orbital implant material