Cellular fatty acid profile distinguishes Burkholderia pseudomallei from avirulent Burkholderia thailandensis |
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| Authors: | Inglis Timothy J J Aravena-Roman Max Ching Simon Croft Kevin Wuthiekanun V Mee Brian J |
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| Affiliation: | Division of Microbiology and Infectious Diseases, Western Australian Centre for Pathology and Medical Research, Nedlands, Western Australia, Australia. tim.inglis@health.wa.gov.au |
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| Abstract: | Burkholderia pseudomallei, the cause of melioidosis, can be distinguished from the closely related but nonpathogenic Burkholderia thailandensis by gas chromatography (GC) analysis of fatty acid derivatives. A 2-hydroxymyristic acid derivative (14:0 2OH) was present in 95% of B. pseudomallei isolates and no B. thailandensis isolates. GC mass spectrophotometry confirmed that 2-hydroxymyristic acid was present in B. pseudomallei. GC-fatty acid methyl ester analysis may be useful in distinguishing these two closely related species. |
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