| C型CpG单链寡核苷酸对哮喘小鼠Th1／Th2相关细胞因子的影响 |
| |
| 引用本文： | 陈敏,吴斌,陈雄. C型CpG单链寡核苷酸对哮喘小鼠Th1／Th2相关细胞因子的影响[J]. 中国临床实用医学, 2009, 3(3): 46-48. DOI: 10.3760/cma.j.issn.1673-8799.2009.03.024 |
| |
| 作者姓名： | 陈敏 吴斌 陈雄 |
| |
| 作者单位： | 广东省湛江广东医学院附属医院呼吸内科,524001 |
| |
| 摘 要： | 目的C型CpG—ODN对哮喘小鼠Th1／Th2相关细胞因子的影响，为CpG-ODN进一步应用提供依据。方法利用人工合成C型的CpG-ODN（ODN-M362），将其注射于哮喘小鼠腹腔，采用酶联免疫吸附法（ELISA）检测注射后小鼠的Th1和Th2细胞因子的含量，分析其有无差异性。结果C型CpG-ODN组BALF中EOS数量，血清IL-4水平明显低于对照组和哮喘组（P〈0．01），血清IFN-γ明显升高于对照组和哮喘组（P〈0．01）。结论C型的CpG-ODN，对哮喘小鼠Th1／Th2失衡有明显的逆转作用。
|
| 关 键 词： | CpG单链寡核苷酸 哮喘 Th1/Th2 |
The effect of type C CpG-ODN on the expression of Th1/Th2 cytokines in a model of asthma |
| |
| CHEN Min,WU Bin,CHEN Xiong. The effect of type C CpG-ODN on the expression of Th1/Th2 cytokines in a model of asthma[J]. China Clinical Practical Medicine, 2009, 3(3): 46-48. DOI: 10.3760/cma.j.issn.1673-8799.2009.03.024 |
| |
| Authors: | CHEN Min WU Bin CHEN Xiong |
| |
| Affiliation: | . (Department of Respiratory Diseases ,Affiliated Hospital of Guangdong Medical College 524001, China) |
| |
| Abstract: | Objective To explore the effect of C types CpG-ODN on the expression of Th1/Th2 cyto-kines in a model of asthma. Methods Asthmatic mice models were established by ovalbumin intraperitoneal in-jection and atomization inhalation. Then the mice were intraperitoneally injected with C types CpG-ODN (ODN-M362), The serum levels of Th1 cytokine IFN-γand Th2 cytokine IL-4 were measured by ELISA. Meanwhile, e-osinophil count peripheral BALF were performed. Results C types CpG-ODN (ODN-M362) inhatitted the ex-pression of Th1 cytokine IFN-γand Th2 cytokine IL-4 in asthmatic mice (P<0.01). Compared with group Aand group B, group C enhanced obviously Th1 cytokine IFN-γ expression, downregulated Th2 cytokine IL-4 ex-pression. Conclusion ODN-M362, a kind of type C CpG-ODN, could correct the imbalance of Th1/Th2 on cy-tokine level. |
| |
| Keywords: | Th1/Th2 |
| 本文献已被 维普 万方数据 等数据库收录！ |
