上海地区弥漫性大B细胞淋巴瘤的生发中心B细胞样型显著偏低

目的 探讨中国上海地区弥漫性大B细胞淋巴瘤(DLBCL)的生发中心B细胞(GCB)样型与非GCB样型的分布以及可能影响因素.方法 应用免疫组织化学EnVision法分析124例来自该院的原发DLBCL中CD10、bcl-6、MUMl、GCET1和FOXP15种蛋白的表达,采用Hans和Choi两种免疫表型分型法进行分型.其中118例应用荧光原位杂交技术检测t(14;18)和bcl-6基因重排情况.结果 使用Hans法对124例DLBCL进行分型,27例(22%)为GCB样型,97例(78%)为非GCB样型.使用Choi法进行分型显示34例(27%)为GCB样型,90例(73%)为非GCB样型(即ABe样型).GCB样型显著低于非GCB样型(P=0.0001).t(14;18)易位仅4例(3%),3例发生于GCB样型中.bcl-6基因重排阳性的病例为46例(39%),高发于GCB样型中.bcl-6基因重排与bcl-6蛋白表达没有明显相关性.结论 中国上海地区DLBCL的GCB样型显著低于非GCB样型,可能与其DLBCL的t(14;18)低水平发生有关.

Prevalence of germinal center B-cell-like and non-germinal center B-cell-like types of diffuse large B-cell lymphoma in Shanghai,China

Objective To study the prevalence of germinal center B-cell-like(GCB)and non-GCB-like types of diffuse large B-cell lymphoma (DLBCL) in Chinese patients. Methods Immunohistoehemical study for CD10.bcl-6,MUM1,GCET1 and FOXP1 was performed on 124 cases of DLBCL from Shanghai,China.The Hans algorithm and Choi algorithm were applied to classify DLBCL into GCB and non-GCB-like lypes.Fluorescence in-situ hybridization(FISH) for t(14;18)and bcl-6 gene rearrangement was also carried out on 118 cases.Results Of the 124 DLBCL cases studied,27 cases (22%)showed a GCB-like type and 97 cases(78%)showed a non-GCB-like type,when using Hans algorithm On the other hand,34 cases(27%)belonged to GCB-like type and 90 cases(73%)belonged to non-GCB-like type when applying Choi algorithm.The prevalence of GCB-like type was significantly lower than that of non-GCB-like type(P:0.0001).Only four cases(3%)were positive for t(14;18),and three of them were classified as GCB-like type.bcl-6 rearrangement was found in 46 cases(39%)and more frequently encountered in the GCB-like type.There is no relationship between bcl-6 gene rearrangement and bcl-6 protein expression.Conclusions The GCB-like type of DLBCL is significantly less common than non-GCB-like type in Chinese population.This phenomenon is possibly related to the low frequency of t(14;18).