成都市858例不育男性精液质量分析

目的分析成都市不育男性精液情况，了解成都地区不育男性精液质量现状。方法用计算机辅助精子分析（CASA）技术，按照《WHO人类精液及精子一宫颈粘液相互作用实验室检验手册》，使用北京伟力公司WLJY9000仪器，对2008年7月-2009年4月到我院生殖与不孕研究所就诊的858例不育男性精液进行分析。结果858例不育男性精液量（2．62±0．94）ml，pI{值（7．45±0．18），精子密度（69．71±56．72）×10^6／ml，精子活率（55．48±25．62）％，a级精子（23．95±14．85）％，a＋b级精子（39．94±19．50）％。其中原发不育490例，继发不育368例；精液正常338例（39．39％），精液异常520例（60．61％），依次为a＋b级异常占62．35％，a级异常占51．17％，活率异常占48．48％，密度异常占17．37％，精液量异常占14．45％，液化异常占8．16％，pH值异常1．28％。结论我市不育男性精液质量与同期育龄男性精液质量相比，精液质量明显下降，表现为60．61％的不育男性患者存在精液质量异常，精子活力异常所占比例最高，提示精液质量下降是导致男性不育的主要因素。多种精子质量检测技术的应用将使男性不育特异性病因诊断变为可能。

Investigation on the sperm quality of 858 males infertility in Chengdu

Objective To investigate the sperm quality of male infertility in Chengdu area. Methods According to 《WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction》 ,whole the 858 semen samples of the male infertility were analyzed through CASA（ WLJY9000, made by BEUING WEILI Company）, and wbole the patients who hospitalized in our hospital were all because of infertility from Jul. 2008 to Apr. 2009. Results Among the 858 males infertility,the semen volume was （2. 62±0. 94） ml,pH value was （7.45±0. 18） ,the sperm concentration was （69.71±56. 72） ×10^6/ ml,tbe sperm viability rate was （55.48±25.62）% ,grade A sperm was （23.95±14. 85）% and the grade A and B sperm was （39. 94±19. 50）%.490 cases were belong to primary sterility group and 368 cases were secondary sterility. The samples with normal sperm quality accounted for 39. 39% （338/858） in contrast with abnormal 60.61% （520/858）. Among the abnormal sampies, there were 535 （62. 35% ）with abnormal grade a＋b,439 （51.17% ）with abnormal gTade a,416 （48.48%）with abnormal viability, 149（ 17.37% ） with abnormal concentration, 124 （ 14.45% ） with abnormal semen volume,70 （ 8.16% ） with abnormal liquefying time and 11 （ 1.28% ） with abnormal pH value. Conclusion compared with the males fertility, the semen quality of the male infertility populaiton in Chengdu area had a marked decreased,520（60. 61% ） males infertlity had an abnormal sperm quality and mostly for abnormal sperm motility. All that show us the drop in the semen quality is a main factor for male infertility, and through the use of various technology on sperm testing, that make the diagnosis of the specific pathogeny for male infertility possible.