alpha1-acid glycoprotein possesses in vitro pro- and antiinflammatory activities |
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Authors: | Pukhal'skii A L Shmarina G V Lyutov A G Novikova L I Shemyakin I G Aleshkin V A |
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Institution: | (1) Methodical-and-Genetic Research Center, Russian Academy of Medical Sciences, Moscow;(2) G. N. Gabrichevskii Moscow Institute of Epidemiology and Microbiology, Moscow;(3) State Research Center of Applied Microbiology, Moscow oblast, Obolensk |
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Abstract: | We studied the effects of 1-acid glycoprotein on tumor necrosis factor- (TNF- ) and interleukin-10 (IL-10) production and lymphocyte response to phytohemagglutinin in cultured peripheral blood mononuclear leukocytes from 6 healthy donors. We observed 2 opposite responses to 1-acid glycoprotein: first, stimulation of TNF- and IL-10 production and inhibition of lymphocyte proliferation, and second, suppression of cytokine production and stimulation of lymphocyte proliferation. In cell cultures isolated from 4 of 6 donors, the TNF- /IL-10 ratio remained unchanged after addition of native 1-acid glycoprotein, but some fractions isolated by chromatography on concanavalin A-Sepharose changed this parameter. These changes were most pronounced after treatment with fraction C enriched with molecules with incomplete (biantennary) carbohydrate chains. The mechanisms of 1-acid glycoprotein-induced effects on peripheral blood mononuclear leukocytes are discussed. |
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Keywords: | 1-acid glycoprotein" target="_blank">gif" alt="agr" align="BASELINE" BORDER="0">1-acid glycoprotein glycoforms tumor necrosis factor- " target="_blank">gif" alt="agr" align="BASELINE" BORDER="0"> interleukin-10 lymphocyte response to phytohemagglutinin |
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