Molecular cloning, expression, and characterization of cyclophilin A from Clonorchis sinensis |
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Authors: | Wu Weihua Chen Jiajia Zeng Suxiang Zhang Zhaoping Gan Wenjia Yu Xinbing Hu Xuchu |
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Affiliation: | (1) Zhongshan School of Medicine, Department of Parasitology, Sun Yat-sen University, Guangzhou, People’s Republic of China;(2) Key Laboratory of Tropical Diseases Control, Ministry of Education, Sun Yat-sen University, Guangzhou, People’s Republic of China; |
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Abstract: | This study described the recognization, cloning, and recombinant expression of cyclophilin A-like gene from Clonorchis sinensis adult complementary DNA library (CsCyPA) and its expression and secretion in adult. Western blotting demonstrated the recombinant CsCyPA could be recognized by sera of clonorchiasis patients and a sole protein of the same size in the excretory-secretory antigens of in vitro cultured adult could be recognized by antiserum raised against the recombinant CsCyPA. Immunohistochemistry demonstrated that the CsCyPA was secreted in scattered vesicles from subtegumental parenchyma cells to the surface of tegument and mainly released from the tegument. ELISA showed the serum levels of IgG against CsCyPA in clonorchiasis patients negatively correlated with worm loads. This study suggested that C. sinensis adult in biliary ducts could release CsCyPA without signal peptide through nonclassical secretory pathway into the liver and might play a role in inflammation and biliary epithelium proliferation and adenomatoid hyperplasia. |
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