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引发条件对干旱胁迫下白花蛇舌草种子萌发及幼苗生长的影响
引用本文:朱再标,卢魏魏,郭巧生,曹亚悦,冯杉,宁梓君. 引发条件对干旱胁迫下白花蛇舌草种子萌发及幼苗生长的影响[J]. 中国中药杂志, 2014, 39(8): 1391-1395
作者姓名:朱再标  卢魏魏  郭巧生  曹亚悦  冯杉  宁梓君
作者单位:南京农业大学 中药材研究所, 江苏 南京 210095;南京农业大学 中药材研究所, 江苏 南京 210095;南京农业大学 中药材研究所, 江苏 南京 210095;南京农业大学 中药材研究所, 江苏 南京 210095;南京农业大学 中药材研究所, 江苏 南京 210095;南京农业大学 中药材研究所, 江苏 南京 210095
基金项目:国家"重大新药创制"科技重大专项(2009ZX09308-002,2012ZX09304006)
摘    要:为了优化白花蛇舌草种子的引发条件,提高白花蛇舌草种子和幼苗的抗旱性,采用均匀设计优化GA3,KNO3,PEG等引发剂的引发浓度和引发时间,引发处理后种子置于15%PEG干旱胁迫下发芽并在1/4 Hoagland培养液中培养30 d。结果表明,366 mg·kg-1 GA3引发1 h显著促进白花蛇舌草种子萌发,提高干旱条件下种子的发芽率、发芽指数、活力指数并促进幼苗的生长;3.0% KNO3引发1 h显著促进白花蛇舌草种子萌发,但对白花蛇舌草幼苗期的抗旱性没有显著影响;PEG对白花蛇舌草种子发芽没有显著的引发效果。适宜浓度的GA3引发处理,可以促进白花蛇舌草种子萌发并增强其幼苗阶段的抗旱性。

关 键 词:白花蛇舌草种子  种子引发  干旱  种子发芽  幼苗
收稿时间:2013-09-18

Impact of priming on seed germination and seedling growth of Oldenlandia diffusa under drought stress
ZHU Zai-biao,LU Wei-wei,GUO Qiao-sheng,CAO Ya-yue,FENG Shan and NING Zi-jun. Impact of priming on seed germination and seedling growth of Oldenlandia diffusa under drought stress[J]. China Journal of Chinese Materia Medica, 2014, 39(8): 1391-1395
Authors:ZHU Zai-biao  LU Wei-wei  GUO Qiao-sheng  CAO Ya-yue  FENG Shan  NING Zi-jun
Affiliation:Institute of Medicinal Plants, Nanjing Agricultural University, Nanjing 210095, China;Institute of Medicinal Plants, Nanjing Agricultural University, Nanjing 210095, China;Institute of Medicinal Plants, Nanjing Agricultural University, Nanjing 210095, China;Institute of Medicinal Plants, Nanjing Agricultural University, Nanjing 210095, China;Institute of Medicinal Plants, Nanjing Agricultural University, Nanjing 210095, China;Institute of Medicinal Plants, Nanjing Agricultural University, Nanjing 210095, China
Abstract:Current study was carried out to optimize the priming condition of Oldenlandia diffusa seeds, and improve germination rate and seed vigor of O. diffusa seeds under drought conditions. Uniform design was used to optimize the concentration and priming time of three priming materials (PEG, KNO3, GA3). Different concentrations of polyethylene glycol (PEG) was used to simulate drought stress. The seedling was cultured in 1/4 Hoagland medium for 30 d. The results showed that seed priming treatment with 366 mg·kg-1 GA3 for 1h resulted in significant increase in germination rate, germination index, vigor, root length, plant height and biomass of O. diffusa seeds under drought stress (15% PEG), while seed priming with 3.0% KNO3 for 1 h showed little effect on germination and growth of O. diffusa seeds under drought stress. Seed priming treatment with appropriate GA3 concentration and priming time could enhance seed germination and drought resistance of O. diffusa in seedling stage.
Keywords:Oldenlandia diffusa  seed priming  drought resistance  seed germination  seedling
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