High-content screening of peptide-based non-viral gene delivery systems |
| |
Authors: | de Raad Markus Teunissen Erik A Lelieveld Daphne Egan David A Mastrobattista Enrico |
| |
Affiliation: | Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Faculty of science, University of Utrecht, Universiteitsweg 99, 3584 CG Utrecht, The Netherlands. |
| |
Abstract: | High-content screening (HCS) uses high-capacity automated fluorescence imaging for the quantitative analysis of single cells and cell populations. Here, we developed an HCS assay for rapid screening of non-viral gene delivery systems as exemplified by the screening of a small library of peptide-based transfectants. These peptides were simultaneously screened for transfection efficiency, cytotoxicity, induction of cell permeability and the capacity to transfect non-dividing cells. We demonstrated that HCS is a valuable extension to the already existing screening methods for the in vitro evaluation of non-viral gene delivery systems with the added value that multiple parameters can be screened in parallel thereby obtaining more information from a single screening event, which will accelerate the development of novel gene delivery systems. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|