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Cellular distribution and degradation of cobalt ferrite nanoparticles in Balb/3T3 mouse fibroblasts |
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| Authors: | Marmorato Patrick Ceccone Giacomo Gianoncelli Alessandra Pascolo Lorella Ponti Jessica Rossi François Salomé Murielle Kaulich Burkhard Kiskinova Maya |
| Institution: | a European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Via E. Fermi 2749, 21027 Ispra (VA), Italy b ELETTRA, Sincrotrone Trieste, Strada Statale 149, 34149 Basovizza, Trieste, Italy c European Synchrotron Radiation Facility (ESRF), 6 rue Jules Horowitz, 38000 Grenoble, France |
| Abstract: | The effect of the concentration of cobalt ferrite (CoFe2O4) nanoparticles (NPs) on their intracellular location and distribution has been explored by synchrotron radiation X-ray and fluorescence microscopy (SR-XRF) monitoring the evolution of NPs elemental composition as well. In cells exposed to low concentrations of CoFe2O4 NPs, the NPs preferentially segregate in the perinuclear region preserving their initial chemical content. At concentrations exceeding 500 μM the XRF spectra indicate the presence of Co and Fe also in the nuclear region, accompanied by sensible changes in the cellular morphology. The increase of the Co/Fe ratio measured in the nuclear compartment indicates that above certain concentrations the CoFe2O4 NPs intracellular distribution could be accompanied by biodegradation resulting in Co accumulation in the nucleus. |
| Keywords: | X-ray microscopy X-ray fluorescence Fibroblasts Magnetic nanoparticles |
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