穿心莲内酯对口腔癌生长的影响

 目的：探讨穿心莲内酯对口腔癌的作用及其机制。方法：用穿心莲内酯及其对照药物治疗金黄地鼠颊囊癌，通过测量肿瘤体积观察治疗效果。用免疫组化检测穿心莲内酯对肿瘤血管和肿瘤细胞增殖的作用，用TUNEL法检测穿心莲内酯对肿瘤细胞凋亡的作用，用免疫组化检测凋亡信号分子caspase-3的表达情况。结果：穿心莲内酯治疗组颊囊癌的肿瘤体积明显比对照组小，肿瘤微血管密度和增殖的肿瘤细胞也明显低于对照组，肿瘤细胞的凋亡率明显高于对照组，而且治疗组中caspase-3明显被活化。结论:穿心莲内酯通过抑制肿瘤血管生成和肿瘤细胞增殖以及促进肿瘤细胞凋亡，抑制了金黄地鼠颊囊癌的生长。穿心莲内酯通过caspase-3途径诱导了肿瘤细胞的凋亡。

Effect of andrographolide on oral squamous cell carcinogenesis

AIM：To study the effect of andrographolide (Andro) on oral squamous cell carcinogenesis. METHODS：Chemical-induced hamster buccal pouch cancer model was used. The tumor cell proliferation, apoptosis and microvessel density (MVD) were detected by immunohistochemical staining. RESULTS：Compared with control group, the tumor volume, MVD and tumor cell proliferation in Andro group were significantly decreased. The cell apoptotic cell number in Andro group was higher than that in control group. Caspase-3 was also activated in Andro group. CONCLUSION:Andro inhibits tumor growth by suppressing proliferation, decreasing MVD and promoting apoptosis in the hamster buccal pouch. Furthermore, Andro promotes tumor cell apoptosis through caspase-3 pathway.