抗内毒素核心糖脂抗体大肠杆菌J5株免疫血清的制备

目的　探讨制备高效价抗内毒素核心糖脂 ( E.Coli J5 )抗体 ,为免疫治疗革兰阴性菌 ( GNB)菌血症和感染性休克奠定基础。方法　应用大肠杆菌 O111:B4变异株 ( J5 )制备无毒菌苗 ( 5 0× 10 1 2 / L )。12只家兔经耳缘静脉注射 J5菌苗 (另 12只用生理盐水作对照 ) ,每 3d注射 1次 ,共 5次 ,每次剂量依次为 0 .1、0 .2、0 .4、0 .6和 0 .8ml。第 5次注射后 1周由心脏取血 4 0～ 5 0 m l,分离血清 ,采用间接血凝法测定抗体效价及交叉反应试验。结果　 12只免疫家兔中 ,6只抗体效价在 1∶ 10 2 4以上 ,并与多种 GNB内毒素产生交叉反应。结论　自制的 E.Coli J5抗体效价高 ,可与多种 GNB内毒素相结合

Anti-endotoxin core glycolipid antibody:the preparation of immune serum of E.Coli J5

OBJECTIVE: To prepare high titer anti-endotoxin core glycolipid (J5) antibody (CGL) for the treatment of Gram-negative bacteremia and septic shock. METHODS: Nontoxic bacterial vaccine (50 x 10(12)U/L) against E.Coli O111:B4 mutant strain J5 was prepared. J5 bacterial vaccine was injected into rabbits through ear marginal vein (saline as control preparation), one time pre three days, totally five times. Injected doses were as following: 0.1 ml, 0.2 ml, 0.4 ml, 0.6 ml, and 0.8 ml. One week after fifth injection, blood samples from heart were collected and immune serum was isolated. Indirect clotting test was used to determine the titer of antibody and cross reaction. RESULTS: Among 12 immunized rabbits, titers of antibody against E. Coli J5 were exceeding 1:1 024 in 6 rabbits, and they had cross reaction with various kinds of Gram-negative bacterial endotoxins. CONCLUSION: The titer of anti-endotoxin core glycolipid (E.Coli J5) antibody prepared by us appears to be high, and it can combine with various kinds of Gram-negative bacterial endotoxins.