OC-3-VGH卵巢癌细胞简捷培养方法的研究

目的：探索简捷细胞培养方法并观察该方法对癌细胞特性的影响。方法：将复苏的OC-3-VGH卵巢癌细胞株不经洗涤直接移至细胞培养瓶，加入10ml RPMI-1640培养液，放入培养箱，余同传统方法，观察细胞生长情况；取细胞悬液，以细胞数4×10^6／ml，0.2ml／只接种至BALB／c雌性裸小鼠皮下，2个月后处死，取肿瘤组织制片，并与传统培养方法结果进行比较。结果：两种方法培养的细胞均生长活跃，皮下接种7d左右，裸鼠长出肿瘤，组织学检查未见明显差异。结论：改良细胞培养方法减少了传统方法的繁琐步骤，方便细胞培养。

study on Cell Culture Method of Ovarian Cancer Cell Line OC-3-VGH

Objective:To explore a modified cell culture method and observe its influences on characteristics of cell line OC-3-VGH of ovarian cancer. Methods:Resuscitated cells of ovarian cancer cell line OC-3-VGH were moved to cell culture bottle directly without washing, and put into incubaton after adding lOml RPMI - 1640 nutrient solution. The other processes were consistent with the traditional method, and the situation of cell growth was observed. Cell suspension (4 × 10<'6>/ml) was taken out and inoculated to BALB/c female nude mice (0.2ml per mouse). All mice were executed after 2 months, and tumor tis-sues were taken out. Tissue sections were prepared and compared with the traditional culture method. Results:Active cell growths were presented in the 2 cell culture methods. Tumor tissues were observed almost at the 7th day after hypodermic inoc-ulation. And there were no significant differences in histological examination. Conclusion:The modified cell culture method of this study is more convenient for cell culture than traditional method with its simple procedures.