热休克蛋白在实验性大鼠脑死亡肾中的表达

目的研究热休克蛋白(HO-1、HSP27和HSP70)在脑死亡大鼠肾中的表达。方法将气囊导管插入F344雄鼠大脑硬膜外慢慢充气,诱导脑死亡,未处理组做对照组,4 h后处死大鼠,采用RT-PCR、蛋白印迹和免疫组化检测肾脏HO-1、HSP27和HSP70的表达。结果RT-PCR显示HO-1(P<0.01)和HSP70(P<0.01)基因编码表达增强。蛋白印迹也显示HO-1蛋白水平增加(P<0.01),但HSP70蛋白表达无明显变化。免疫组化显示HO-1蛋白在肾皮质表达增强。HSP27在脑死亡供体肾的表达无变化。结论脑死亡引起的肾应激可诱导细胞保护基因HO-1和HSP70的表达。

Renal expression of heat shock proteins in experimental rats with brain death

Objective To study renal expression of the genes HO-1,HSP27 and HSP70 in rats with brain death.Methods Male F344 rats were given brain death by inflating air with balloon catheter to epidural space.normal rats were control group.they were killed 4 hours after brain death.the expression of the genes HO-1,HSP27 and HSP70 were tested by RT-PCR,Western blotting technique and immunohistoche mistry.Results RT-PCR showed an increase in expression of genes coding of HO-1(P<0.01) and HSP70(P<0.01) after brain death.Western blotting also revealed an increase in HO-1 protein levels(P<0.01) but no change in HSP70 protein expression.Immunohistoche mistry showed an increse of HO-1 protein expression in the renal cortical tubules in brain-death rats.no changes were observed in renal HSP27 expression after brain death.Conclusion The expression of the cytoprotective genes HO-1 and HSP70can be induced by renal stress caused by brains death.