乙肝病毒血清标志物与PCR检测HBV—DNA的关系

为了探讨ＰＣＲ技术在乙肝病毒感染诊断中的应用及ＨＢＶ－ＤＮＡ与ＨＢＶＭ间的关系，对５２４例乙肝病毒感染者的血清采用ＰＣＲ与ＥＬＩＡＳ进行检测。结果：ＨＢｓＡｇ、抗－ＨＢｃ、ＨＢｅＡｇ三项阳性组，ＨＢＶＭ全阴组，单项抗－ＨＢｓ阳性组，ＨＢＶ－ＤＮＡ检出率分别为８０．１４％、４７．６２％、２６．４７％，提示三项阳性组ＨＢＶ－ＤＮＡ检出率是８组形式中的最高组，标志病毒复制活跃、传染性强、ＨＢＶＭ全阴组也

Relationship Between Seral Markers of Infection with HBV and the Detection of HBV-DNA by PCR Technique

To invertigate the possible application with hepatitis type B viruo as well as the relationship between HBV-DNA and HBVM,the serum of 524 patients infected with hepatitis typeB virus was detcted by using PCR and ELIAS techmques. The results indicate that the detection rates are 80.14% (131/141), 47.6% (20/42) and 26.47% (9/34) respectively in Group Awhere HBsAg, anti-HBC, and HBeAg are all positive; Group B where HBVM is negativeand Group C where only anti-HBs is positive it is suggested that the highest detection rate ofHBV-DNA falls into Group A, indicating that the duplication of the virus in active andhighly infections Negative HBVM in Group B does not rule out the possibility of infectionwith hepatitis Type-B virus. It is possible that there exists chronic hepatitis in Group CPCR delection of HBV-DNA enriches nad renew the knowledge of infection with HBV.However, ELISA detection of HBVM as initial screening is indispensible for expressing thenon-duplicating state of HBV