放射性药物与Egr-Endostatin重组质粒的协同抑瘤作用

目的探讨Egr-内皮抑素基因联合放射性药物-胶体32P对于大鼠肝癌的抑制效果。方法经局部引入基因重组质粒及胶体32P后于,7 d1、4 d2、1 d分别测肝癌的生长率,第21d测定肝癌微血管密度(MVD)、肿瘤细胞凋亡指数(AI)等指标的变化。结果各干预组的肿瘤体积生长率、MVD、AI与对照组指标相比均具有统计学差异,P均0.05-0.01,在所有干预组中,32P+Egr-Endostatin组的抑瘤效果最好,P均0.05-0.01。肝癌生长率方面,第7 d-14d,32P干预组与单纯内皮抑素基因组、Egr-内皮抑素基因组相比抑瘤效果相近,P0.05;但到达21 d时,胶体32P干预组治疗效果则明显下降。基因联合胶体32P的干预组效果均好于其它干预组,P0.05-0.01。MVD方面,32P组与其它干预组比较具有统计学差异,P0.01。AI指标,其他干预组与32P组比较具有差异,P0.05。结论 32P联合Egr-Endostatin基因协同抑瘤,可以发挥放射性药物的照射作用,Eg-1的辐射诱导增强作用及Endostatin基因的抑制肿瘤新生血管的三重作用,疗效最为确切。

Radioactive Drugs and Egr-endostatin Synergistic Antitumor Effect

Objective To investigate the antitumor effect of Egr-Endostatin gene combined with radioactive drug - colloidal I inhibitory on the rat liver. Methods After gene and colloidal 32 p were injected into liver cancer, the growth rate of liver cancer were measured at 7 d, 14 d, 21 d . Microvessel density determination （MVD）and apoptosis index （A I） were be measured at 21 d. Results Compared with the control the indexs in the treatment groups bad significant difference index（ P 〈 0.05 - 0.01 ）. The inhibitory effect of ^32 p ＋ Egr-Endostatin is the best（ P 〈 0.01 ）. In the term of liver cancer growth rate, there are not significant difference between the ^32p group, Endostatin gene and Egr-Endostatin gene group in antitumor effect at 7 d - 14 d, （ P 〉 0.05） ; But at 21 d, the treatment effect of ^32 p group is clearly down. In terms of MVD, compared with the control group the treatment group has obvious statistical difference （ P 〈 0.01 ） ; compared with the other treatment group,^32p group has statistical difference（ P 〈 0.01 ） ;^32 p ＋ Endostatin group has statistical comparing with other groups（ P 〈 0.01 ）. In tems of AI index, the control group has statistical difference comparing with treatment groups （ P 〈 0.01 ） ;^32p group also has difference comparing with Endostatin group and Egr-Endostatin group （ P 〈 0.05） ; AI index of ^32 p ＋ Endostatin group and ^32 p ＋ Egr-Endostatin is better than other treatment groups（ P 〈 0.05 - 0.01 ）. Conclusion Colloidal ^32 p combining Egr-Endostatin gene plays the important role in treating liver cancer. ^32p can create the radiation function, Egr-1 can induce expression enhancement of Endostatin and the Endostatin Gene can inhibit liver ttanor angiogenesis. Therefore the whole effect of the three factors can manifestly decrease the liver cancer growing.