落新妇苷在小鼠体内的组织分布

目的 建立落新妇苷组织浓度的高效毛细管电泳分析方法,并对其在小鼠体内的组织分布进行测定.方法 高效毛细管电泳之胶束电动模式测定小鼠尾静脉注射30 mg/kg落新妇苷后不同时间点各组织中药物含量.结果 落新妇苷在组织器官的浓度分布特点是:C心＞C肝＞C肾＞C脾＞C肺＞C脑.心脏组织C5min＞C15min＞ C60min,自5 min浓度达峰后呈缓慢递减趋势,C60min仍保持较高浓度.其他组织C15min达峰,肝脏在C15min至C60mm均保持较高药物浓度,C60 min在各组织中浓度最高.60 min内脑组织含有较稳定药物含量.结论 落新妇苷在小鼠体内分布广泛,心、肝、肾保持较高组织浓度,且能够穿越血脑屏障分布到脑组织中.

Tissue distribution of astilbin in mice

Objective To establish a high performance capillary electrophoresis(HPCE) method for analysis of tissue distribution of astilbin in mice.Methods The model of micellar electrokinetic capillary chromatography(MECC) of HPCE was adopted after intravenous injection.The concentrations of astilbin in biological samples were determined. Results The tissue distribution feature of astilbin was C_heart > C_liver > C_kidney > C_spleen > C_lung > C_brain. The Cheart shows C_{5 min} > C_{15 min} > C_{60 min} and C_{5 min} is C_max. But C_{15 min} was C_max in other tissues. The steady content of astilbin was found in brain tissue within 60 min. Conclusion Astilbin was widely distributed in main tissues, which could also permeate blood brain barrier.