A study of norepinephrine metabolism in rat brain using a double label technique |
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Authors: | Fountaine C Brown John S Zawad |
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Institution: | Alcohol and Drug Research Center, Departments of Psychiatry and Biochemistry, University of Tennessee Center for the Health Sciences, Memphis, TN, U.S.A. |
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Abstract: | Using a double-label procedure, the incorporation of endogenously-derived 35SO4 into phenylethylene glycol sulfates (PGS) was estimated. When 3H]norepinephrine and 35S]cysteine were injected concomitantly into the brain, about 30–80 percent of the tritium and about 4 percent of the 35S retained in the brain 1 hr later were in PGS. In B6-deficient rats, the proportion of 35S was increased as was the ratio. Probenecid caused a significant increase in the amount of PGS found in the brain, but a minimal enrichment of 35S was observed, suggesting that there was little or no effect on sulfotransferases. Cysteine in high concentrations inhibits the incorporation of tritium into PGS. |
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