The relative ethylumbelliferone dealkylase activity characterizing the effect of different inducers on the hydroxylase system in the liver musomes of female mice |
| |
Authors: | Gertrud Mohn Petra Niederlaender Claudia Kessler |
| |
Affiliation: | Department of Physiological Chemistry, University of the Saarland, 6650 Homburg/Saar, German Federal Republic |
| |
Abstract: | d,l-Camphor was detected as a new inducer of hydroxylase in the liver musomes of female mice. After a 2-day inhalation of d,l-camphor, cyt. P-450 and the ethylumbelliferone dealkylase were increased by 250 per cent and the NADPH-cyt. P-450 reductase by 350 per cent. The product [NADPH-cyt. P-450 reductase activity × cyt. P450 concentration] was shown to be a suitable reference parameter for the ethylumbelliferone dealkylase activity in the liver musomes during the treatment with four different inducers. The relative dealkylase activity Q was much decreased during inhalation of cyclohexane or d,l-camphor.Obviously these two inducers preferably enhanced cyt. P-450 species with a low dealkylase activity. The Q-values were reproducible. Q was increased by 100 per cent during induction of a MC-sensitive mouse strain with 3-methylcholanthrene, but it was only moderately decreased by induction with phenobarbital. Corresponding to this, methylcholanthrene is known to selectively induce a cyt. P-448 with high dealkylase activity whereas phenobarbital is known to change the hydroxylase specificity in the liver musomes not very much. |
| |
Keywords: | (used mainly in the figures) EU = ethylumbelliferone CH = cyclohexane PB = phenobarbital MC = 3-methylcholanthrene K = controls fed only 2 hr/day V = mice with freely accessible food |
本文献已被 ScienceDirect 等数据库收录! |