In vitro translation products of turnip crinkle virus RNA |
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Authors: | Altenbach S B Howell S H |
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Institution: | Department of Biology, C-016, University of California, San Diego, La Jolla, California 92093, USA. |
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Abstract: | Turnip leaves infected with turnip crinkle virus accumulate a 35-kd polypeptide which comigrates with the major protein from isolated virions. RNA from TCV virions directs the synthesis of a number of polypeptides in vitro including a 35-kd protein which is immunoprecipitable with antiserum to virus particles. Translation of viral RNA size-fractionated on sucrose gradients or methyl mercurichydroxide-containing gels shows that the TCV coat protein is synthesized primarily from RNA fragments which are smaller than the genomic RNA. A satellite RNA species found in virions does not direct the synthesis in vitro of any identifiable protein. |
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Keywords: | To whom reprint requests should be addressed |
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