特比萘芬与伊曲康唑和氟康唑体外联合抗白念珠菌的实验分析

目的评价特比萘芬、伊曲康唑和氟康唑体外对28株甲真菌病白念珠菌的相互作用方式。方法参照美国国家实验室标准委员会(NCCLS)M27方案(1997)推荐的棋盘微量稀释法,检测了28株分离自甲真菌病患者的白念珠菌体外对特比萘芬分别与伊曲康唑和氟康唑联合的药物敏感性试验。结果特比萘芬与伊曲康唑联合用药时,17.9%(5/28)受试菌株表现为协同作用方式,71.4%(20/28)的受试菌株表现为相加作用方式,10.7%(3/28)受试菌株表现为无关作用方式,未观察到拮抗作用的出现;特比萘芬与氟康唑联合用药时,17.9%(5/28)受试菌表现为协同作用方式,71.4%(20/28)的受试菌株表现为相加作用方式,10.7%(3/28)受试菌株表现为无关作用方式,未观察到拮抗作用的出现。结论特比萘芬与三唑类药物对甲真菌病白念珠菌分离株存在有效的相互作用。

Activities of Terbinafine in Combination with Itraconazole and Fluconazole Against Isolates of Candida albicans In Vitro

Objective To evaluate the interaction of terbinafine with either itraconazole and fluconazole against 28 clinical isolates of Candida albicans from onychomycosis patients in vitro. Methods A checkerboard microdilution method that adhered to the recommendations of the National Committee for Clinical Laboratory Standards(NCCLS-M27)was applied to study the vitro sensitivity of 28 clinical isolates of Candida albicans to the combination therapy of terbinafine with either Itraconazole and Fluconazole and their interactions in vitro. Results When terbinafine and itraconazol were given in combination,synergy was observed in 17.9%(5/28)of the isolates and 71.4%(20/28)of the isolates were additive,and 10.7%(3/28) were indifferent,while antagonism was not observed.When terbinafine and fluconazole were given in combination,synergy was observed in 17.9%(5/28)of the isolates and 71.4%(20/28)of the isolates were additive,and 10.7%(3/28) were indifferent,while antagonism was not observed.Conclusion An effective interaction between terbinafine and triazoles against isolates of Candida albicans from onychomycosis patients.