大肠上皮性良恶性病变花生凝集素受体的研究

本文应用 ABC 酶标记亲合组织化学技术和图象定量分析手段,对大肠良恶性病变的花生凝集素受体(PNA-R)进行了组化染色和定量研究。结果显示:正常结肠粘膜上皮缺乏 PRN-R,阳性例数为10%,定量 B/A 值为0.010,而大部分大肠癌组化染色显示PNA-R 阳性.未经神经氨酸酶消化,阳性例数为66%。经神经氨酸酶消化,阳性例数为79%,和正常结肠粘膜上皮比较,二者具非常显著性差异,并且 PNA-R 的分布发生了明显改变,正常结肠粘膜分布于核上区,即高尔基体区;而大肠癌 PNA-R 大部分分布于细胞顶端或腺腔的腔面,该分布形式和胎儿结肠 PNA-R 分布形式相似,定量分析也证实大肠癌 PNA-R 明显增多;腺瘤、幼年性息肉、慢性炎症显示 PNA-R 阳性率有所增高,但分布形式和正常结肠粘膜相同。由此我们认为如果 PNA-R 量明显增多,分布形式发生改变,应警惕癌变的发生.

STUDIES ON PENUT AGGLUTININ RECEPTOR IN BENIGN AND MALIGNANT DISEASES OF LARGE INTESTINE FROM EPITHELIUM

The paper has studied penut agglutinin receptor(PNA-R)in large intestinal benign and malignant diseases with avidin-biotin-peroxidase complex method and image analysis system.The results showed that normal mucosa lacks PNA-R.In ten cases of normal adult mucosa,only one expressed PNA-R at the supranuclear portion of the cells. 58 cases of different types of colorectal cancer,without prior neuraminidase treatment, 66% of the cancer was positive and localized PNA-R to the apical cytoplasmic region of the cells.After prior neuraminidase treatment,positive rate was increased up to 79%.Com- pared with normal mucosa,the positive rate was highly significantly different(P<0.01). The results of rate and location of PNA-R in eight fetal colonic mucosa examined was sim- ilar to cancer.38% of tubular adenomas,57% of villous-tubular adenomas,50% of juve- nile polyps and 33% of chronic inflammation expressed PNA-R at the supranuelear por- tion of the cells.The results of quantitative analysis showed that B/W(B:positive area, W:negtive area)and B/A(A=B W) was increased and that different groups was highly significantly different(P<0.01).we have same results by using two kinds of method.This finding provids a technique for us to distinguish colonic cancer from normal mucosa and other benign lesions.If we find that the position and quantity of PNA-R of colonic mucosa change,we will pay attention to malignant transformation.