Two new multi-purpose multicopy Schizosaccharomyces pombe shuttle vectors,pSP1 and pSP2 |
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| Authors: | Guillaume Cottarel David Beach Ulrich Deuschle |
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| Institution: | (1) Cold Spring Harbor Laboratory, Howard Hughes Medical Institute, 11724 Cold Spring Harbor, NY, USA;(2) Present address: Basel Institute for Immunology, Grenzacherstrasse 487, CH-4005 Basel, Switzerland |
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| Abstract: | Plasmids pSP1 and pSP2 are two new Schizosaccharomyces pombe ars1 multicopy vectors with the Saccharomyces cerevisiae LEU2 and URA3 genes as selectable markers. They are derivatives of S. cerevisiae integrative plasmids. These plasmids allow classical molecular genetic techniques, such as mutagenesis, nested deletions and sequencing, to be performed directly. |
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| Keywords: | Autonomously replicating sequence Recombinant DNA Nested deletions Mutagenesis Schizosaccharomyces pombe |
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