Differential expression of protein kinase C subtypes during ginsenoside Rh2-induced apoptosis in SK-N-BE(2) and C6Bu-1 cells |
| |
Authors: | Young Sook Kim Sung Ha Jin You Hiu Lee Jong Dae Park Shin Il Kim |
| |
Affiliation: | Korea Ginseng and Tobacco Research Institute, Youseong-Gu, Taejon. yskim@gtr.kgtri.re.kr |
| |
Abstract: | We examined the modulation of protein kinase C (PKC) subtypes during apoptosis induced by ginsenoside Rh2 (G-Rh2) in human neuroblastoma SK-N-BE(2) and rat glioma C6Bu-1 cells. Apoptosis induced by G-Rh2 in both cell lines was confirmed, as indicated by DNA fragmentation and in situ strand breaks, and characteristic morphological changes. During apoptosis induced by G-Rh2 in SK-N-BE(2) cells, PKC subtypes alpha, beta and gamma were progressively increased with prolonged treatment, whereas PKC delta increased transiently at 3 and 6 h and PKC epsilon was gradually down-regulated after 6 h following the treatment. On the other hand, PKC subtype zeta markedly increased at 24 h when maximal apoptosis was achieved. In C6Bu-1 cells, no significant changes in PKC subtypes alpha, gamma, delta, epsilon and zeta were observed during apoptosis induced by G-Rh2. These results suggest the evidence for a possible role of PKC subtype in apoptosis induced by G-Rh2 in SK-N-BE(2) cells but not in C6Bu-1 cells, and raise the possibility that G-Rh2 may induce apoptosis via different pathways interacting with or without PKC in different cell types. |
| |
Keywords: | Ginsenoside Rh2 Apoptosis Protein kinase C SK-N-BE(2) cells C6Bu-1 cells |
本文献已被 PubMed SpringerLink 等数据库收录! |
|