多指标综合评价复方大黄灌肠液提取工艺研究

目的：优选复方大黄灌肠液提取工艺。方法：基于大黄灌肠液组方原则，兼顾芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚和枸橼酸主要指标成分含量和水溶性总煎出物，以加水量、浸泡时间、提取时间和提取次数为考察因素，采用多指标综合评分法，利用正交试验优选复方大黄灌肠液提取工艺条件。结果：最佳提取工艺为加水量为6倍，浸泡10 min，提取60 min，提取3次。结论：该工艺合理可行，稳定可控，可用于制剂的实际生产。

Study on Extraction Techniques of Compound Rhubarb Enema by Multi-index Comprehensive Evaluation

This study was aimed to optimize extraction techniques of Compound Rhubarb Enema. Based on the formula principle of Compound Rhubarb Enema, effects of the amount of water, soaking time, extraction time and frequency on each index were studied. The content of Aloe-emodin, Rhein, Emodin, Chrysophanol, Physcion, Citri cacid, and water soluble substance were taken into consideration. The best extraction condition was optimized by using the orthogonal test coupled with the multi-index comprehensive evaluation. The results showed that the best condition was soaking for 10 min, extraction time for 60 min, extraction for 3 times; and 6 times of water was added. It was concluded that this preparation techniques were feasible and reliable for the manufacturing process of Compound Rhubarb Enema.