活血及活血解毒配伍中药对急性心肌梗死大鼠模型血小板活化、炎症反应及凝血状态的影响

目的观察活血及活血解毒配伍中药对急性心肌梗死大鼠血小板活化、炎症反应及凝血状态的影响。方法 100只SD雄性大鼠,按随机数字表法分为假手术组、模型组、活血组、活血解毒组、倍他乐克组,每组20只。活血组给予芎芍胶囊（0.39 g/kg）,活血解毒组给予芎芍胶囊（0.39 g/kg）加黄连胶囊（0.135 g/kg）,倍他乐克组给予倍他乐克片（2.25 mg/kg）,假手术组及模型组均给予等量生理盐水,连续干预3周。末次灌胃后,利用在体结扎冠状动脉前降支法建立急性心肌梗死模型,假手术组只穿线不结扎。造模24 h后处死动物,用ELISA法检测血清中β-血小板球蛋白（β-TG）、血小板α颗粒膜蛋白-140（GMP-140）、11去氢血栓烷B2（11-DH-TXB2）、纤维蛋白肽A（FPA）,抗凝血酶Ⅲ（AT-Ⅲ）、D-二聚体（DD）及肿瘤坏死因子α（TNF-α）指标,采用RT-PCR法检测大鼠心肌TNF-αmRNA表达。结果与假手术组比较,模型组血小板活化指标（β-TG、11-DH-TXB2及GMP-140）均明显升高（P〈0.01）;与模型组比较,除倍他乐克组GMP-140外,各给药组血小板生化指标均有明显降低（P〈0.01,P〈0.05）,且活血解毒组β-TG、11-DH-TXB2水平优于倍他乐克组（P〈0.05）,而活血解毒组11-DH-TXB2亦优于活血组（P〈0.05）。凝血状态显示：与假手术组比较,AMI模型组高凝状态明显,FPA、D-D水平升高（P〈0.05,P〈0.01）,抗凝的ATⅢ降低（P〈0.01）;与模型组比较,各给药组FPA水平明显降低（P〈0.01）,活血及活血解毒组ATⅢ明显升高（P〈0.01）,活血解毒组D-D明显降低（P〈0.01）,且活血解毒组3项指标均优于倍他乐克组（P〈0.05）。TNF-α血清含量及心肌TNF-αmRNA表达显示：与假手术组比较,模型组血清TNF-α含量及心肌组织TNF-α表达明显升高（P〈0.05,P〈0.01）;与模型组比较,活血解毒组不仅TNF-α的血清含量降低（P〈0.01）,同时心肌组织中TNF-α的基因表达也

Effect of Activating Blood Circulation or Activating Blood Circulation and Detoxication on Platelet Activation,Inflammation,and Coagulation Status in Acute Myocardial Infarction Rats

Objective To observe the effect of activating blood circulation drugs or activating blood circulation and detoxication drugs on indices of platelet activation,inflammation,and coagulation status correlated with blood-stasis and toxin in acute myocardial infarction rats. Methods Totally 100 male SD rats were randomly divided into the sham-operation group,the model group,the activating blood circulation group,the activating blood circulation and detoxication group,and the metoprolol group,20 in each group. Rats in the activating blood circulation group were administered with Xiongshao Capsule at the daily dose of 0. 39 g / kg. Rats in the activating blood circulation and detoxication group were administered with XiongshaoCapsule（ at the daily dose of 0. 39 g / kg） and Huanglian Capsule（ at the daily dose of 0. 135 g / kg）. Rats in the metoprolol group received metoprolol at the daily dose of 2. 25 mg / kg. And rats in the rest two groups were administered with normal saline. All medication lasted for 3 successive weeks. After the last administration,the rat model of acute myocardial infarction was prepared by ligation of left anterior descending artery. No ligation was given to rats in the sham-operation group. Animals were sacrificed 24 h after modeling. Tumor necrosis factor-α（ TNF-α）,β-thromboglobulin（ β-TG）,platelet α granule membrane protein-140（ GMP-140）,11 dehydro-thromboxane B2（ 11-DH-TXB2）,fibrinopeptide A（ FPA）,antithrombinⅢ（ AT-Ⅲ）,and D-dimer（ DD） were detected by ELISA. The mRNA expression of TNF-α was tested by RT-PCR. Results Platelet activation parameters were significantly increased in the model group,when compared with the sham-operation group（ P 〈0. 01）. Compared with the model group,all indices（ except GMP-140 in the metoprolol group） obviously decreased in each medicated group（ P 〈0. 01,P 〈0. 05）. Besides,β-TG and 11-DH-TXB2 were superior in the activating blood circulation and detoxication group to that of the metoprolol group（