| 龟板含药血清体外诱导成年大鼠骨髓间充质干细胞分化为神经元 |
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| 引用本文： | 陈东风,杜少辉,李伊为,黎辉.龟板含药血清体外诱导成年大鼠骨髓间充质干细胞分化为神经元[J].广州中医药大学学报,2003,20(3):224-226. |
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| 作者姓名： | 陈东风 杜少辉 李伊为 黎辉 |
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| 作者单位： | 1. 广州中医药大学解剖教研室,广州,510405
2. 广东省深圳市中医院内科,深圳,518033 |
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| 基金项目： | 国家自然科学基金项目(编号:30271677) |
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| 摘 要： | 【目的】观察龟板含药血清体外诱导成年大鼠骨髓间充质干细胞分化为神经元的能力。【方法】采用龟板含药血清体外定向诱导第五代骨髓间充质干细胞，免疫组化鉴定神经丝蛋白(NFP)、胶质纤维酸性蛋白(GFAP)的表达。【结果】成年大鼠骨髓间充质干细胞受龟板血清诱导后神经元样细胞NF表达阳性，诱导后12h，神经元样细胞NF阳性表达达到高峰。【结论】龟板含药血清可以在体外诱导成年大鼠骨髓间充质分化为神经元。
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| 关 键 词： | 龟板 体外诱导 成年大鼠 骨髓间充质干细胞 细胞分化 神经元 超微结构 含药血清 |
| 文章编号： | 1007-3213(2003)03-0224-03 |
| 修稿时间： | 2003年5月14日 |
In-Vitro Induction of Mesenchymal Stem Cells Differentiation into Neurons in Adult Rats by Serum Containing Carapax et Plastrum Testudinis |
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| CHEN Dongfeng,DU Shaohui,LI Yhuei,LI Hui.In-Vitro Induction of Mesenchymal Stem Cells Differentiation into Neurons in Adult Rats by Serum Containing Carapax et Plastrum Testudinis[J].Journal of Guangzhou University of Traditional Chinese Medicine,2003,20(3):224-226. |
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| Authors: | CHEN Dongfeng DU Shaohui LI Yhuei LI Hui |
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| Abstract: | Objective] To observe the effect of serum containing Carapax et Plastrum Testudinis (CPT) in inducing the differentiation of mesenchymal stem cells (MSC) of adult rats into neurons. Methods] The fifth generation of MSC were induced by the serum containing CPT (sero-CPT) and the expressions of neurofilament (NF) protein and glial fibrillary acidic protein (GFAP), the marks of neuron-like cells were detected by immunohistochemical method. Results] The expression of NF protein was positive after the stimulation of sero-CPT and the expression reached the peak 12 hours after induction. Conclusion] Sero-CPT can induce the differentiation of MSC into neurons in vitro. |
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| Keywords: | CARAPAX ET PLASTRUM TESTUDINIS/phaimacology BONE MARROW CELLS/ ultrastructure STROMAL CELLS/ ultrastructure NEURONS/ultrastructure CELL DIVISION/drug effects |
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